HPLC法同时测定益骨颗粒中3种成分的含量

Simultaneous Determination of Three Components in Yigu Granules by HPLC

目的建立同时测定益骨颗粒中淫羊藿苷、龙胆苦苷、马钱苷酸3种成分含量的方法。方法采用高效液相色谱法(HPLC),色谱柱为Agilent Eclipse Plus C_(18)(250 mm×4.6 mm,5μm),流动相为乙腈-0.1%磷酸溶液(梯度洗脱),流速为1.0 mL·min^(-1),检测波长为254 nm,柱温为25℃,进样量为10μL。结果淫羊藿苷、龙胆苦苷、马钱苷酸3种成分的检测质量浓度线性范围分别为4.072~101.8,20.252~506.3,4.044~101.1μg·mL^(-1)(r=0.999 9),平均回收率分别为99.51%、98.74%、98.77%,RSD分别为1.16%、1.02%、1.79%(n=6)。结论该测定方法操作简便、结果准确、重复性及专属性好,可用于益骨颗粒的质量控制。

OBJECTIVE To establish a method for simultaneous determination of icariin, gentiopicroside and loganic acid in Yigu granules. METHODS HPLC was performed on the column of Agilent Eclipse Plus C18（250 mm×4.6 mm,5 μm） with mobile phase ofacetonitrile-0.1% phosphoric acid （gradient elution） at a flow rate of 1.0 mL. min-1, the detection wavelength was 254 nm, column temperature was 25℃, and the injection volume was 10 p,L. RESULTS The linear range was 4.072-101.8 μg-mL-1 for icariin（r=0.999 9）, 20.252-506.3 μg.mL-1 for gentiopicroside （r=0.9999）, 4.044-101.1 μg.mL-1 for loganic acid （r=0.999 9）, respectively. The average recoveries were 99.51% （ RSD = 1.16%, n= 6）, 98.74% （ RSD = 1.02% ,n = 6）, 98.77% （ RSD = 1.79% ,n= 6）, respectively. CONCLUSION The method is simple and accurate with good repeatability and specificity, and could be used for the quality control of Yigu granules.