活细胞非标记检测技术

Label-free imaging for living cell detection

活细胞成像技术可以实时或在一定时间内观察细胞内部结构和生理过程，能够提供比固定细胞成像技术更加真实可靠的信息。现有的活细胞成像技术多基于荧光成像技术，通过增加荧光基团亮度、减少细胞毒性来获得高信噪比的图像，这些方法对细胞活性会造成一定的影响。现代物理技术的改进使得活细胞非标记成像检测成为可能，本文就活细胞的非标记物理成像技术进行深入探讨，以期为活细胞的非标记检测提供新的方法参考。

The living cell imaging technique can observe the internal structure and physiological processes of living cells in real time or a period of time, which can provide more reliable information compared with the fixed cell imaging method. Most of the existing techniques for living cell imaging are based on fluorescence imaging technology, which through increasing brightness of the fluorescent groups to reduce cytotoxicity and obtain high signal to noise ratio of the image. These kinds of methods will cause certain influence on the activity of the cells. The improvement of modern physical technology makes label- free imaging method for living cell detection become possible. This paper reviews the current state of label- free imaging methods for living cell detection which may provide a new method for living cell detection.