黑果枸杞果实成熟发育过程表达谱差异分析

Differential Expression Analysis of Gene Expression Profiles During Fruit Development and Ripening of Lycium ruthenicum

对黑果枸杞(Lycium ruthenicum Murr.)3个不同发育时期的果实进行转录组测序,分析对比黑果枸杞果实不同发育时期相关基因表达谱的变化。提取果实RNA进行Illumina高通量测序,利用GO、KEGG等公共数据库进行功能注释、分类,利用数字基因表达谱技术对比分析。结果显示,KEGG pathway富集分析表明亮氨酸生物合成途径在变色期对比青果期有7个差异表达基因,黑熟期对比变色期有35个差异表达基因,且全部为上调表达。在糖酵解途径中3个不可逆的反应步骤限速酶基因在黑熟期均为上调表达。对黑果枸杞亮氨酸合成和糖酵解涉及到的基因进行了功能和代谢通路的分析,为黑果枸杞种质资源探索提供理论基础。

The purpose of this study was to sequence the transcriptome at 3 different developmental stages of Lycium ruthenicum Murr.fruit,as well as analyze and compare the variations of their gene expression profiles. The RNAs of the fruits were extracted,and sequenced byIllumina high-throughput,then the their functions were annotated and classified using public database such as GO,KEGG,etc.,further theirgenes were compared and analyzed by digital gene expression profiles. Enrichment analysis results by KEGG pathway showed that in the ＂leucinebiosynthesis pathway＂,there were 7 genes differentially expressed in color changing vs early light green skin stage,while 35 differentiallyexpressed genes in full ripening stage vs color changing stage,and all were up-regulated. The gene expressions of 3 rate-limiting enzymes inthe non-reversible reactions of glycolysis all were up-regulated in the full ripening stage. Moreover,the functions and metabolic pathways of allgenes involved in leucine biosynthesis and glycolysis of L. ruthenicum fruit were analyzed,aiming at providing the theoretical basis for exploringthe germplasm of L. ruthenicum.