bFGF改善白内障手术引发角膜内皮损伤

Effect of basic fibroblast growth factor on corneal endothelial cell damage after cataract surgery

目的探究碱性成纤维细胞生长因子(b FGF)对白内障手术引发角膜内皮损伤修复的作用。方法采用超声乳化摘除30只兔子(60眼)的晶体,将其分为2组,随机分为bFGF治疗组和对照组。bFGF治疗组滴用bFGF眼用凝胶,对照组滴用相同体积的生理盐水,分别每天滴用3次。观察记录2组眼睛消肿的时间;在手术后不同时间,分别检测2组切口的愈合面积、角膜厚度、角膜内皮细胞密度,分别取各组前房水,检测各组前房水中NO、IL-1、IL-6和TNF-α的含量。结果 bFGF治疗组的眼睛消肿时间较对照组显著减少(P<0.05);bFGF治疗加速了切口愈合速率、角膜厚度和角膜内皮细胞密度的恢复及前房水内NO、IL-1、IL-6和TNF-α的减少。结论 bFGF可改善白内障手术引起的角膜内皮细胞损伤。

Objective To explore the effect of basic fibroblast growth factor （bFGF） on corneal endothelial cell damage after cataract surgery. Methods Thirty rabbits （60 eyes） whose lens extraction were done by phacoemulsification were divided into 2 groups, namely, bF- GF treatment group and control group, bFGF ophthalmic gel was used to treat the eyes 3 times daily in bFGF treatment group, and the saline of the same volume was used to treat the eyes in control group. The time of corneal edema subsidence was recorded;the healing area of inci- sion, corneal thickness, the density of corneal endothelial cells and the NO, IL-1, IL-6 and TNF-ct contents of the aqueous sample in anterior chamber were measured. Results The time of corneal edema subsidence of bFGF treatment group decreased significantly compared with con- trol group;bFGF treatment accelerated the healing rate of incision, the recovery of corneal thickness and the density of corneal endothelial cells and the decrease of the NO, IL-1 IL-6 and TNF-ct contents of the aqueous sample in anterior chamber. Conclusion bFGF treatment can accelerate the recovery of the corneal endothelial cell damage,which was induced b- cataract surgery.