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基于顶空固相微萃取/气质联用的红茶特征香型呈香活性成分研究 被引量:21

Comparison of odor-active components of two different odor type ‘Black Tea'based on HS-SPME / GC-O-MS
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摘要 香气是茶叶(Camellia sinensis(L.)O.Kuntze)感官品质的一项关键因子,本研究拟对红茶"花香"、"甜香"香型的关键活性成分进行分析与鉴定。采用高效液相色谱法等对两种香型红茶的理化成分进行分析比较,发现"花香型"红茶的茶黄素含量(0.367%±0.021%)显著高于"甜香型"红茶(0.274%±0.019%)。采用顶空固相微萃取/气相色谱-嗅香-质谱联用(HS-SPME/GC-O-MS)技术,对两种香型的呈香活性成分进行分析,共鉴定得到44种香气组分,其中19种为呈香活性成分。芳樟醇(花香)、苯甲醛(草药味)、顺式-芳樟醇氧化物(熟土豆味)、(反,反)-2,4-庚二烯醛(辛臭味)等6种成分的呈香强度显著,是两种香型红茶的主要呈香活性成分。同时,水杨酸甲酯(香草味)、橙花醇(花香)、苯甲醛等7种呈香成分是决定两种香型差异的关键因子。本研究揭示了两种特征香型红茶的呈香因子和理化成分差异,为相关茶叶香气化学研究提供借鉴。 The aroma is a key factor for the sensory quality of tea ( Camellia sinensis(L.) O. Kuntze), the key active components of "floral-odor" and "sweet-odor" Black Tea were analyzed and identificated by this study. High performance liquid chromatography and other ways were used to analyzing and comparing the physical and chemical components of two odor-type Black Tea,the content of theaflavins in the floral-odor black tea and the sweet-odor black tea were 0.367% ± 0.021% ,0.274 ± 0.0]9%, respectively.Headspace solid phase microextraction coupled with gas chromatographymass spectrometry and olfactory detector port ( HS- SPME/GC- O- MS) was used to analyzing the aroma-active compounds.A total of 44 volatile components were identified in the two odortype black teas, and ]9 of them were further determined as aromaactive components.6 components got very strong sniffer such as linalool ( floral), benzaldehyde ( herbal ), (Z) - linalool oxide ( furanoid ) ( potato- like odor), (E, E) -2,4- heptadienal ( bugs- like odor), and were the main aromaactive components of Black Tea . Furthermore,7 kinds of compounds were probably the key chemicals contributing to the different perceived aroma of the two odor-type Black Tea, such as methyl salicylate ( herbal), nerol ( floral ), benzaldehyde. This study revealed the aromatic factor and physical and chemical composition difference of the two characteristic odor -type Black Tea, and could considerably offer references for relevant tea aroma studies.
出处 《食品工业科技》 CAS CSCD 北大核心 2016年第23期304-310,共7页 Science and Technology of Food Industry
基金 浙江省自然科学基金资助(LQ14C160003)
关键词 红茶 呈香活性成分 GC-O-MS 花香 甜香 Black tea aroma- active component GC-O- MS floral odor sweet odor
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