丹酚酸B壳聚糖纳米粒制备与大鼠心脏缺血/再灌注损伤保护作用研究

Preparation of Salvianolic Acid B Chitosan Nanoparticles and Their Protective Effects Against Heart Ischemia/Reperfusion Injury in Rats

目的:制备丹酚酸B壳聚糖纳米粒,并考察对大鼠心脏缺血/再灌注损伤的保护作用。方法:采用离子凝胶化法制备丹酚酸B壳聚糖纳米粒,并以包封率(EE%)和粒径分布(nm)为评价指标,对丹酚酸B壳聚糖纳米粒处方进行优化;采用Malvern粒度仪测定纳米粒的粒径分布和Zeta电位,透射电镜考察其形态;并考察丹酚酸B壳聚糖纳米粒的体外释药行为;考察丹酚酸B壳聚糖纳米粒对大鼠心脏缺血/再灌注损伤的保护作用。结果:丹酚酸B壳聚糖纳米粒的包封率为85.8%±3.1%;粒径为(166.1±42.4)nm,Pd I为(0.189±0.032),Zeta电位为(+24.9±4.5)m V;透射电镜显示丹酚酸B壳聚糖纳米粒粒径均一,成球状;纳米粒在24 h内平稳缓慢释药;丹酚酸B壳聚糖纳米粒可以增加大鼠心脏缺血/再灌注损伤的保护作用。结论:丹酚酸B壳聚糖纳米粒对大鼠心脏缺血/再灌注损伤具有良好的保护作用。

Objective：To prepare the salvianolic acid B chitosan nanoparticles and study their protective effects against heart ischemia/reperfusion injury in rats. Methods：The salvianolic acid B chitosan nanoparticles were prepared by iron gelation method. Taking the encapsulation efficiency（EE%）and particle size distribution（nm）as indicator,the formulation variables were optimized. The optimized chitosan nanoparticles were characterized for encapsulation efficiency,particle size distribution,Pd I,zeta potential,morphology,and in vitro drug release behavior of salvianolic acid B chitosan nanoparticles were studied. The salvianolic acid B chitosan nanoparticles protected against heart ischemia/reperfusion injury in rats were studied. Results：For encapsulation efficiency,particle size distribution,Pd I and zeta potential of salvianolic acid B chitosan nanoparticles were found to be（85.8%±3.1%）,（166.1±42.4）nm,（0.189±0.032）and（24.9±4.5）m V,respectively. The nanoparticles were found to be small and spherical as seen in transmission electron microscopy. The in vitro release data proved that the drug release from formulation was steady during 24 h. Salvianolic acid B chitosan nanoparticles could increase protected effectively against heart ischemia/reperfusion injury in rats. Conclusions：Salvianolic acid B chitosan nanoparticles protected effectively against heart ischemia/reperfusion injury in rats.