加工番茄离体再生体系研究

Study on Regeneration System of Processing Tomato in vitro

为建立一套优化的适于加工番茄遗传转化的高效离体再生体系,以加工番茄石红303的子叶、下胚轴、叶片作为外植体,研究了不同激素及浓度、不同外植体材料对愈伤组织诱导、不定芽分化及生根的影响。结果表明:激素的种类和浓度对加工番茄外植体出愈率的影响没有差异,但是诱导形成的愈伤组织形态和不定芽存在差异。选取子叶作为外植体,培养基组成为MS+6-BA3.0mg·L-1+IAA0.2mg·L-1最有利于愈伤组织和不定芽的形成;不定芽在生根培养基1/2MS+0.1mg·L-1 IAA上生根效果最佳,并能发育成完整的小植株。

In order to establish a good kind of system suitable for the hereditary and translation of the processing tomato,cotyledons,hypocotyls and true leaf of processing tomato cultivar Shihong303 were used as explants and cultured on the medium with different hormones and concentrations to study their effect on callus induc- tion, bud differentiation and radication. The results showed that callus could be induced in all kinds medium, but there were obvious differences on callus configuration and bud differentiation. Taking cotyledons as explant, MS＋ 6-BA3.0 mg·L^-1 ＋ IAA0.2 mg·L^-1 was suitable for callus induction and adventitious bud differentiation; The regenerated buds could develop normally after roots induced on 1/2MS＋ IAA 0.1 mg·L^-1.