球根海棠金正日花高效繁殖体系的建立

Establishment of Highly Efficient Propagation System of Begonia tuberhybrieda Vosa

为促进秋海棠金正日花的规模化生产,以球根海棠(Begonia tuberhybrieda Vosa)金正日花的叶片为外植体,研究植物细胞分裂素6-BA、ZT、TDZ和生长素NAA不同种类和浓度对金正日花试管苗增殖的影响。结果表明:最适诱导培养基是MS+6-BA1.5mg·L^(-1)+NAA0.2mg·L^(-1),愈伤组织诱导率达100%,不定芽诱导数达7.2;最佳丛生芽分化培养基为MS+6-BA1.5mg·L^(-1)+NAA0.2mg·L^(-1),增殖系数达8.32;最适生根培养基是MS+NAA0.3mg·L^(-1),生根率为100%。

In order to promote the large-scale production of Begonia tuberhybrieda Vosa, taking the leaves of Begonia tuberhybrieda Vosa as explant, different concentrations of kinetin, 6-BA, ZT,TDZ and NAA was add ed to MS medium to optimize the culture medium ,and the tissue culture and plant regeneration were studied. The optimal medium for inducing callus was MS- 6 BA1.5 mg. L 1 ＋ NAA0.2 mg·L^-1 , which the inductivity could reach to 100% ,abventitious bud induction coefficient achieved at 7.2. The shoots specialized perfectly on medium of MS＋6-BA1.5 mg·L^-1＋NAA0.2 mg·L^-1 ,for the growth coefficient achieved at 8.32. The best me dium for rooting was MS＋ NAA0.3 mg. L 1, which rooting rate approached to 100%.