电针水沟穴对脑缺血再灌注大鼠自噬相关蛋白p62表达的影响

Effect of Electroacupuncture at Shuigou(GV26) on Expression of Autophagy-related Protein p62 after Cerebral Ischemia Reperfusion Injure in Rats

目的:探讨电针水沟穴对大鼠脑缺血再灌注(MCAO/R)损伤的保护作用及与自噬相关蛋白p62的关系。方法:线栓法复制大鼠局造性脑缺血再灌注模型。采用Bederson神经功能缺损体征评分法对MCAO/R大鼠进行神经功能评分;Western blot检测MCAO/R大鼠自噬相关蛋白p62的表达。结果:与假手术组比较,模型组大鼠有明显神经功能缺损体征,自噬相关蛋白p62的表达明显下调;与模型组相比针刺治疗组大鼠神经功能缺损体征明显减轻,自噬相关蛋白p62的表达明显上调。结论:电针水沟穴可能是通过拮抗自噬相关蛋白p62表达的下调减轻脑缺血再灌注损伤。

Objective：To study the protective effect of electro- acupuncture at Shuigou（GV26） on cerebral ischemia referfusion injure in rats and discuss the relationship with autophagy -reiated Protein p62. Methods：Focal cerebral ischemia referfusion rats were induced by middle cerebral artery occlusion with thread embolus. By using of Bederson score method, the rats＇ neurological deficit scores were observed after MCAO/R injure. The level of autophagy - related Protein p62 was investigated by Western Blot. Results ： The results suggest that the model group had obvious signs of neurological deficit and the expressions of autophagy - related Protein p62 were significantly decreased in comparison to that of the sham group. The results also showed that the neuro- logical deficit scores in treatment group were markedly decreased and the expressions of Autophagy - related Protein p62 were sig- nificantly increased in comparison to that of the model group. Conclusion：These results suggest that electroacupuncture at Shuigou （GV26） protects brain against ischemia reperfusion injury in rats through inhibiting the low -regulation of autophagy -related protein p62.