氟咯草酮对大鼠原代培养支持-生精细胞氧化应激的影响

Effect of fluorochloridone on oxidative stress in primary co-cultured sertoli-germ cell of rat

目的探讨氟咯草酮对大鼠原代培养支持-生精细胞的影响及可能机制。方法使用二步酶消化法建立大鼠原代支持-生精细胞共培养体系，接种于细胞培养板培养24h后分别用10-6、10-7、10-8mol／L的氟咯草酮染毒培养液和溶剂对照培养液孵育24h，分别进行MTT实验和氧化应激指标检测。结果10-6mol／L氟咯草酮染毒组原代大鼠支持-生精细胞体系细胞死亡率较对照组和10-7、10-8mol／L染毒组明显增高，差异有统计学意义（P〈0．05）。10-6mol／L氟咯草酮染毒组过氧化氢酶、超氧化物歧化酶、总谷胱甘肽过氧化物酶活力降低，总谷胱甘肽含量下降、丙二醛含量升高，与对照组和10-7、10-8mol／L染毒组的差异均有统计学意义（P〈0．05）；10-7mol／L氟咯草酮染毒组过氧化氢酶活力较对照组下降，超氧化物歧化酶活力较10-8mol／L染毒组降低，总谷胱甘肽含量较对照组和10-8mol／L染毒组减少，差异均有统计学意义（P〈0．05）。结论氟咯草酮对原代大鼠睾丸支持-生精细胞可产生毒性损害作用，其机制可能与对睾丸支持-生精细胞的氧化应激有关。

Objective To explore effect offluorochloridone on primary co-cultured sertoli-germ cell of rat and its possible mechanism. Methods primary co-cultured sertoli-germ cell was made by two steps of enzyme digestion with SD rat testes, after 24h of Sertoli-germ cell isolation, A 0.1% DMSO solvent control group and three FLC exposure groups （10-6,10-7,10-8 mol/L） were selected, cultured cell for 24h, then MTT assay and index detection of oxidative stress were performed. Results The mortality of primary co-cultured sertoli-germ cell exposed to 10-6 mol/L FLC was significantly higher than control group and FLC exposure groups （ 10-7, 104 mol/L） （P〈0.05）. 104 mol/L FLC reduced enzyme activity of CAT, SOD and GSH. Px, depressed GSH level, elevated MDA level,and had significant difference than control group and FLC exposure groups （10-7,10-8 mol/L）（P〈 0.05 ）. 10-7 mol/L FLC decreased enzyme activity of CAT than control group, depressed enzyme activity of SOD than 10-8 mol/L FLC ,lowered GSH level than control group and FLC exposure groups （10-8 mol/L）, and had statistical difference （P〈0.05）. Conclusion FLC can damage primary co-cultured sertoli-germ cell of rat, its possible mechanism is relevant to oxidative stress.