焦炉作业工人外周血淋巴细胞周期分布与尿中多环芳烃代谢物的关系

Relationship between urinary polycyclic aromatic hydrocarbon metabolite and cell cycle of lymphocyte in coke oven workers

目的探讨焦炉作业工人外周血淋巴细胞周期分布与尿中多环芳烃代谢物的关系。方法选择437名焦炉作业工人为焦炉组，163名水处理工人为对照组，用密度梯度离心法分离淋巴细胞，以流式细胞分析仪检测焦炉组和对照组工人的淋巴细胞细胞周期分布，用酶水解的方法检测尿中多环芳烃代谢产物的含量。结果焦炉组尿中2-羟基萘、2-羟基芴、9-羟基菲和1-羟基芘含量明显高于对照组，差异有统计学意义（P〈0．01）。以各代谢产物含量小于疡为低接触组，以含量大于％为高暴露组对两组工人的细胞周期进行分析，结果显示，高接触组S期细胞所占比例明显高于低接触组，差异有统计学意义（z=-2．496，P=0．013），高接触组G0／G1期细胞所占比例明显低于低接触组，差异有统计学意义（Z=-2．074，P=0．038）。以其他羟基代谢产物作为内暴露分组，各期细胞所占比例差异无统计学意义（P〉0．05）。结论尿中1-羟基芘含量增高可导致淋巴细胞周期紊乱，主要表现为G0／G1期缩短，S期阻滞。

Objective To investigate the relationship between urinary polycyclic aromatic hydrocarbon metabolite and cell cycle of lymphocyte in coke oven workers. Methods 437 coke oven workers and 163 workers in water treatment department were recruited in this study. Flow cytometry was used to detect the cell cycle of lymphocyte. For the measurement of urinary metabolites, urine samples were treated with [3-glucuronidase and analyzed using HPLC with a fluorescence detector. Results The concentrations of urinary 2-naphthol, 2- hydroxyfluorene, 9-phenanthrol and 1-hydroxypyrene 1 in coke oven workers were significantly higher than those in control group （P〈0.01）. The distributions of cell cycle were analyzed in high exposure group （the content of urinary metabolites high than P75 ） and low exposure group （the content of urinary metabolites low than/}25 ）. According to the content of 1-hydroxypyrene, the proportions of S phase in high exposure group were significantly higher than those of low exposure group （Z=-2.496,P=0.013）, but the proportions of G0/G1 phase were significantly lower than low exposure group （Z=-2.074,P=0.038）. The similar results were not been found in other hydroxylated metabolites as internal exposure group. Conclusion Increasing levels of urinary 1- hydroxypyrene might resulting in cell cycle of lymphocyte disorders, mainly for G0/G1 phase shorten and S phase arrest.