芒果果实转录组数据组装及基因功能注释

Transcriptome Data Assembly and Gene Function Annotation of Mango Fruits

以红芒6号为试材,对生长发育各时期果皮与果肉样品提取RNA后等量混合进行转录组测序,共获得了68 419 722个reads,包含6 157 744 980个核苷酸序列信息,平均读长90 bp,序列信息全部登陆NCBI数据库(SRP035450)。对reads进行拼接,共获得124 002个Contig片段,进一步组装获得54 207个Unigene片段,平均长度为838 bp,阈值小于10-5的序列中共有42 515(78.43%)个unigenes被注释到公共蛋白数据库。其中,35 198个被注释到生物学过程、分子功能和细胞组分GO类别的44个功能组,14 619个Unigene匹配到25类COG功能组,23 741个Unigene被富集到128个KEGG代谢通路,包括代谢途径、次生代谢的生物合成、植物-病原物互作、植物激素信号转导、苯丙氨酸生物合成、淀粉和蔗糖代谢、类黄酮类化合物合成等。本研究将为进一步的芒果功能基因克隆、基因表达分析、分子标记开发等研究奠定基础。

We performed transcriptome sequencing of mixed RNA isolated from fruit sample for ‘ Zill＇ mango（ Mangifera indica Linn） including pericarp and pulp during different development stage using Illumina RNA-seq technology. RNA-seq generated 68 419 722 sequence reads encompassing 6 157 744 980 total nucleotides（ nt）,each sequence read averaging 90 bp in length. All the sequence read datasets were deposited at the NCBI Sequence Read Archive（SRA）（Gen Bank accession SRP035450）. All high-quality reads were assembled into 124 002 contigs.these contigs were further assembled into 54 207 unigene with a mean size of 838 bp. A total of 42 515（78.43%）unigenes were annotated using public protein databases with a cut-off E-value above 10-5. out of these, 35 198 unigenes and 14 619 unigenes were assigned to Gene Ontology terms and Clusters of Orthologous groups.Functional annotation against Kyoto Encyclopedia of Genes and Genomes pathway database identified 23 741（ 43.79%） unigenes which were mapped to 128 pathways, which including Metabolic pathways, Biosynthesis of secondary metabolites, Plant-pathogen interaction, Plant hormone signal transduction, Phenylpropanoid biosynthesis,Starch and sucrose metabolism, Flavonoid biosynthesis. This study will lay a foundation for further research on functional gene cloning, gene expression analysis and molecular marker development in mango.