Notch受体在胰腺癌组织中的表达及其临床意义

Notch Receptor Expression and its Clinical Significance in Pancreatic Cancer

目的:探讨胰腺癌及癌旁组织中Notch1～4蛋白和mRNA表达及临床意义。方法:应用免疫组化方法检测40例胰腺癌组织及对应的癌旁组织石蜡标本中Notch1～4蛋白的表达情况;分析Notch1～4受体表达水平与胰腺癌临床病理特征关系;应用实时逆转录聚合酶链反应(Real-time RT PCR)检测冰冻胰腺癌组织及对应的癌旁组织手术标本中Notch1～4的mRNA表达情况。结果:免疫组化结果显示:Notch1、Notch2在胰腺癌组织中蛋白表达明显比胰腺癌旁组织低(P<0.05);Notch3在胰腺癌组织中蛋白表达比胰腺癌旁组织高,但是差异无统计学意义(P>0.05);Notch4在胰腺癌组织中蛋白表达比胰腺癌旁组织高(P<0.05)。Notch1～4受体的表达与肿瘤分化程度和临床分期呈负相关(P<0.05)。Real-time RT PCR结果显示:与胰腺癌旁组织相比,癌组织中Notch1,Notch2的mRNA相对表达水平显著下调;胰腺癌癌组织中Notch3,Notch4的mRNA相对表达水平显著上调。结论:Notch家族的Notch1～4受体在胰腺癌组织及其旁组织的蛋白和mRNA表达不同,Notch蛋白表达可以作为胰腺癌的风险预测因子。

Objective： To explore the protein and mRNA expression of Notch1--4 in pancreatic carcinoma and adjacent tissues. Methods： The expression of Notchl--4 protein in 40 pairs of paraffmembedded pancreatic specimens and adjacent tissues were detected by immunohistochemistry. The relationship between the protein expression and clinicopathological characters was analyzed. The mRNA expression of Notchl--4 in 40 frozen pancreatic carcinoma and adjacent tissues were detected by Real-time RT PCR. Results： The protein expression of Notchl and Notch2 was lower in pancreatic carcinoma compared with that in adjacent pancreatic tissues （%2=-9.872, 8.672, P〈0.05）; The protein expression of Notch3 was higher in pancreatic carcinoma compared with that in adjacent pancreatic tissues, but the difference was not statistically significant. The protein expression of Notch4 was higher in pancreatic carcinoma compared with that in adjacent pancreatic tissues （P〈0.05）; The expression of Notch receptor had a negative correlation with tumor differentiation and clinical stage （P〈0.05）; Compared with adjacent pancreatic tissues, the mRNA expression of Notchl and Notch2 were down-regulated,Notch3 and Notch4 were up-regulated in pancreatic carcinoma. Conclusion： The protein and mRNA expression of Notch 1--4 of Notch receptor family were different inpancreatic carcinoma and adjacent tissues. The expression of Notch receptor may be used as a predictor of clinical screening and risk of pancreatic cancer.