DEK:一种新的乳腺癌筛查和预后评估的指标

DEK:A Novel Early Screening and Prognostic Marker for Breast Cancer

目的:探讨DEK在乳腺癌中的表达和临床意义。方法:收集628例乳腺癌患者的临床病理标本,采用Real-time PCR、免疫组化染色及蛋白印迹法检测乳腺癌和癌旁组织中DEK的表达,分析DEK与乳腺癌临床病理参数及预后的关系。结果:DEK在乳腺癌组织中的表达显著高于癌旁组织,其与乳腺癌病理分级、淋巴结转移、远处转移、肿瘤大小相关(P均<0.01)。此外,DEK蛋白高表达的乳腺癌患者的OS和DFS比DEK低表达的患者差。DEK蛋白高表达在早期、中晚期均对乳腺癌预后的判断具有评估价值,高表达的DEK乳腺癌患者与DEK蛋白低表达患者相比,总生存及无病生存时间均明显减少(P<0.001)。74.30%(185/249)乳腺癌远处转移病例表现出DEK高表达,53.83%(204/379)的非远处转移例表现出DEK高表达(P<0.001)。结论:乳腺癌中DEK基因高表达与远处转移显著相关。乳腺癌中DEK基因高表达与不良预后显著相关。DEK基因表达情况是评估乳腺癌预后的独立指标。DEK基因高表达与化疗耐药有一定的相关性。DEK基因是潜在的预测乳腺癌患者预后的一项生物标志物。

Objective： The present study aimed to investigate the expression status and clinical implications of DEK in breast cancer. Methods： DEK expression status was detected in 628 breast cancer specimens by Real-time PCR, western blot analysis and immu-nohistochemistry staining, and the correlation between DEK protein and clinicopathological parameters and prognosis of breast cancer was subsequently determined. Results： In comparison to paracarcinoma tissues, DEK protein was highly expressed in breast cancer specimens and was correlated with chemo-therapy resistance. According to universal analysis, it was observed that age, tumor size, histological grade, metastatic nodes and distant metastasis （P〈 0.001） are key factors associated with DEK. Furthermore, compared with samples with no or low DEK protein expression, high DEK expression resulted in a significantly increased distant metastasis rate and poor disease specific survival （P=0.001）. In addition, DEK protein was detected as an independent prognostic factor （P=0.001） in the Cox regression analysis. Conclusions： DEK was correlated with chemotherapy resistance and may be an independent prognostic factor for breast cancer, as well as a potential therapeutic target.