人间充质干细胞的无异源培养体系研究进展

Advances in Xeno-free Culture Systems of Human Mesenchymal Stromal Cells

人间充质干细胞具有自我更新和多向分化的潜能,越来越多的应用于细胞再生医学领域。临床应用细胞制剂的制备,需要在GMP指导下对人间充质干细胞进行体外扩增。传统的方法人间充质干细胞扩增方法含FBS,但由于存在批次间质量差异、异源污染和免疫排斥风险,无异源培养方法显得尤为必要。多种替代FBS的人源性血液成分已经在研究之中,如血清、血浆和血小板衍生物等。化学成分限定性培养基是标准化的最终目标,目前已有商业化的培养基上市。无异源培养基是临床细胞分离和扩增的前提,可保证临床细胞治疗应用的安全性和有效性,是该领域走向产业化的必然趋势。本文对人间充质干细胞的无异源培养体系研究进展做一综述。

Mesenchymal Stem/Stromal cells （MSCs） have both self-renewal and differentiation potential and are increasingly applied in cell-based regenerative medicine. To yield clinically relevant cell doses, ex vivo expansion of MSCs is required to be compliant with good manufacturing practice （GMP） guidelines. Traditional protocols still use fetal bovine serum （FBS） to expand MSCs. However, the high lot-to-lot variability, risk of contamination and immunization call for xenogenic-free culture conditions. Various human blood-derived components to replace FBS are under investigation, including human serum, human plasma and human platelet derivatives. Chemically defined media are the ultimate achievement in terms of standardization, some commercial chemically de？ned media have been on the market. The use of xeno-fxee media is a prerequisite for clinical MSC isolation and expansion, also can guarantee the safety and efficacy of clinical cell therapy application, and has inevitable trend of industrialization. In this paper, we provide a brief review on research progress in xeno-free culture systems of MSCs.