皮氏蛾螺和水泡蛾螺线粒体基因组及核糖体ITS2的比较研究

Comparison of Complete Mitochondrial Genomes and ITS2 Regions in Whelks Volutharpaperryi and Buccinium pemphigum

利用PCR技术分别扩增皮氏蛾螺和水泡蛾螺的线粒体全基因组以及核糖体第二转录间隔区ITS2,对所得序列进行了分析。结果表明,皮氏蛾螺和水泡蛾螺的线粒体基因组全序列长度分别为15 255bp、15 265bp,具有相似的碱基组成和AT含量,其基因排列顺序完全一致,蛋白质编码基因长度相同,两种螺的ITS2分别为340bp和329bp,一致性达83.6%。经比对发现,皮氏蛾螺和水泡蛾螺的线粒体基因组及ITS2序列与已知的新腹足目动物相似。利用线粒体全基因组和核糖体序列构建系统发育树,两种序列的分析均显示皮氏蛾螺和水泡蛾螺亲缘关系较近。

In this study, we amplified the complete mitochondrial genome and ITS2 nucleotides in whelks Volutharpa perryi and Buccinium pernphigurn. After the whelks were sequenced and assembled, the soft- ware Bioedit 7.0.1, Mega 5 and Phylip-3. 695 were used to analyze the data. The complete mitogenome was 15 255 bp in V. perryi and 15 265 bp in B. pemphigum in length, similar in composition and AT content, and identical in gene order. The ITS2 sequences of V. perryi and B. pemphigurn were 340 bp and 329 bp in length, with the identity of 83.6%. By comparison, both mitogenome and ITS2 sequences were found to be similar to most known Neogastropoda animals. Phylogenetic trees were constructed by the complete mitogenome and ITS2 sequences, showing a close relationship between the two species.