益气活血中药对脑出血大鼠GAP-43和Arc的影响

The effect of Yi Qi Huo Xue TCM preparation on the growth of GAP-43 and Arc in intracerebral hemorrhage rat

目的:观察益气活血中药制剂对脑出血大鼠脑组织GAP-43和Arc的影响。方法:用自体血注入法建立大鼠脑出血模型,分空白组、阳性组和实验组。阳性组造模1 d后开始腹腔注射神经节苷脂;实验组造模1 d后用益气活血中药制剂灌胃。分别于干预后1 d、3 d、7 d处死大鼠,用定量PCR法检测各组大鼠脑组织GAP-43和Arc的水平。结果:干预后第7天阳性组与实验组大鼠脑组织GAP-43和Arc含量均高于空白组,差异有统计学意义;第3天实验组GAP-43含量高于阳性组,与阳性组比较差异有统计学意义。结论:益气活血中药制剂对大鼠脑出血损伤后神经结构重建有促进作用,但具体作用机制尚需进一步研究。

Objective： To observe the effect ofYiqi Huoxue TCM preparation on GAP-43 and Arc in intracerebral hemorrhage rats. Method： Intracerebral hemorrhage model rats were established by autologous blood injection and were divided into 3 groups： the blank group, the positive group and the experimental group. The positive group received intraperitoneal injection of ganglion glycosides 1 d after modeling and the experimental group received Yiqi Huoxue Chinese medicine preparation to fill the stomach 1 d after modeling. Rats were killed 1 d, 3 d, and 7 d after intervention respectively, the contents of GAP-43 and Arc level in rat brain tissue were detected by quantitative PCR method. Result： 7 d after intervention, the contents of GAP-43 and Arc level in rat brain tissue of the the positive group and the experimental group were higher than that of the blank group, the difference was of statistical significance; 3 d after intervention, the contents of GAP-43 of the experimental group were higher than that of the positive group, the difference was of statistical significance. Conclusion： Yiqi Huoxue TCM preparation could promote the reconstruction of neural structure after cerebral hemorrhage in rats, but the concrete mechanism needed further research.