溶血反应对临床生化检验结果的影响分析

Influence of Hemolysis on Clinical Biochemical Test Results

目的:研究分析溶血反应对临床生化检测结果的影响。方法:收集我院2015年1月至12月120例正常人体检血液标本资料,采用控制变量法,在试管1中放入正常血液,试管2中放入发生溶血反应血液,分别检测两组血液临床生化指标,并比对变化数据。结果:在检测的17项数据中,血糖、总胆红素、直接胆红素、谷草转氨酶、谷丙转氨酶、碱性磷酸酶、总蛋白、甘油三酯、尿酸、总胆固醇、乳酸脱氢酶等11项数据在试管1与试管2中,检测指标变化大,具有统计学意义(P<0.05)。白蛋白、肌酐、尿酸氮、血钙、酸性磷酸酶、血磷等六项指标在试管1与试管2中,含量检测基本无变化(P>0.05)。结论:溶血反应的发生对临床生化检测结果影响很大,发生溶血反应会直接影响到酶类等指标的检测结果的准确性,临床检测中应尽量避免溶血反应。

Objective： To analyze the influence of hemolysis on clinical biochemical test results. Methods： 120 cases of normal health examination blood specimens were collected in our hospital, and using control variable method, in the normal blood was added into the test tube 1, and the blood with hemolytic reaction was added into the test tube 2, and the clinical biochemical indexes of blood were detected and compared in the two groups. Results： In 17 detected indexes, the difference had statistical significance in comparison of the level of blood glucose, total bilirubin, direct bilirubin, aspartate transaminase, glutamic pyruvic transaminase, alkaline phosphatase,total protein, triglycerides, uric acid, total cholesterol（TC） and lactate dehydrogenase between tube 1 and tube 2（P〈0.05）, and another six indexes including albumin, creatinine, uric acid nitrogen, calcium, acid phosphatase, serum phosphorus had no significant different change between tube 1 and tube 2（P〉0.05）. Conclusion： Hemolytic reaction has great effect on the clinical biochemistry detection results, which can directly affect the accuracy of the results of serum enzymes and other indicators, so it should be to avoid hemolysis reaction in clinical examination.