断颈交感神经干大鼠DO中牵张区NE/adrb3的表达与MSCs动员的关系

Effect of sympathetic denervation on alternation of NE/adrb3 expression and MSCs distribution in rat distraction osteogenesis model

目的:检测断颈交感神经干后牵张成骨(distraction osteogenesis,DO)牵张区去甲肾上腺素(NE)/β3肾上腺素能受体(adrb3)及间充质干细胞标志物Nestin的表达情况,并分析其间的相关性。方法:SD成年雄性大鼠20只,平均分为4组(n=5)。实验组行右侧下颌骨牵张器植入术复合颈交感神经干离断术,对照组行右侧下颌骨牵张器植入术,分别于牵张期开始和牵张期结束处死动物并取材,免疫组织化学检测各组NE/adrb3的表达。应用SPSS13.0软件包对数据进行组间t检验。结果:NE/adrb3主要表达于血管周。与对照组相比,断交感神经后NE/adrb3随着时间延长,表达逐渐减弱,到牵张期结束已无明显表达;实验组Nestin在牵张期开始及结束时,均广泛表达于成骨基质和血管周,且主要集中于成骨基质,而对照组Nestin主要表达于血管周。结论:牵张成骨(DO)中交感神经通过NE/adrb3反向调控间充质干细胞(MSCs)动员及向成骨基质迁移。

PURPOSE： To determine the ahernation of NE/adrb3, Nestin expression in rat model after sympathetic deneration during distraction osteogenesis. METHODS： According to different intervention and time point, 20 adult male SD rats were divided into 4 groups （n=5） equally. Rats in the experimental group underwent right mandible distraction osteogenesis and transection of cervical sympathetic trunk （TCST）, while rats in the control group only underwent right mandible distraction osteogenesis. The right mandibles and the distraction zone specimens were harvested for immunohistochemistry assays of NE/adrb3 expression and mesenchymal stem cells （MSCs） distribution at specific time point. The data were analyzed using SPSS 13.0 software package. RESULTS： The expression of NE was hardly found, and very weak adrb3 expression was observed around the blood vessel in TCST group, but the expression of NE and adrb3 was abundant in the control group; the Nestin＋ MSCs were abundant and mainly distributed in callus matrix in TCST group, whereas that Nestin＋ MSCs were mainly located adjacent to perivascular region in the control group. CONCLUSIONS： TCST depleted sympathetic mediator NE and its receptor adrb3 in distraction zone; Nestin＋ MSCs have higher tendency of migration from perivascular region to callus matrix under distraction stress after TCST in vivo.