高效液相色谱法同时测定壮骨方煮散中朝藿定C和淫羊藿苷含量

Content Determination of Epimedin C and Icariin in Zhuanggufang Zhusan by HPLC

目的建立同时测定壮骨方煮散中抗骨质疏松有效成分朝藿定C和淫羊藿苷含量的高效液相色谱（HPLC）法。方法采用高效液相色谱法,色谱柱为Plastisil C18柱（250 mm×4.6 mm,5μm）,以乙腈-水（27∶73）为流动相,流速为1.0 m L/min,检测波长为270 nm,柱温为30℃。结果朝藿定C进样量在0.050-2.016μg范围内与峰面积线性关系良好（r=0.999 9）,淫羊藿苷进样量在0.032-1.280μg范围内与峰面积线性关系良好（r=0.999 9）;朝藿定C和淫羊藿苷平均加样回收率分别为98.94%和100.99%,RSD分别为1.69%和1.07%（n=6）。结论该方法简便,重复性和稳定性良好,适用于壮骨方煮散中朝藿定C和淫羊藿苷的质量控制。

Objective To establish an HPLC method for the content determination of active ingredients（epimedin C and icariin）of antiosteoporosis in Zhuanggufang Zhusan.Methods HPLC was used with the Plastisil C_（18） column（250 mm × 4.6 mm,5 μm）,acetonitrilewater（27：73） as the mobile phase at a flow rate of 1.0 mL/min,the detecting wavelength of 270 nm,and the column temperature at30 ℃.Results The good linear was over the range of 0.050-2.016 μg for epimedin C（r=0.999 9） and the average recovery（n=6） was 98.94%with RSD of 1.69%;the good linear was over the range of 0.032-1.280 μg for icariin（r=0.999 9） and the average recovery（n=6） was 100.99%with RSD of 1.07%.Conclusion The method is simple and with high repeatability and stability,which can be used for the quality control of Zhuanggufang Zhusan.