高效液相色谱法测定巴戟口服液中淫羊藿苷含量

Content Determination of Icariin in Baji Oral Liquid by HPLC

目的建立测定巴戟口服液中淫羊藿苷含量的高效液相色谱（HPLC）法。方法色谱柱为Syncronis C18柱（250 mm×4.6 mm,5μm）,流动相为乙腈-水（30∶70）,体积流量为1.0 m L/min,柱温为30℃,检测波长为270 nm。结果淫羊藿苷进样量在0.010 2-0.101 5μg范围内与峰面积线性关系良好,平均加样回收率为99.38%,RSD=1.51%（n=6）。结论该方法简便、准确、重复性好,可有效控制巴戟口服液的质量。

Objective To establish an HPLC method for the content determination of icariin in Baji Oral Liquid.Methods The Syncronis C18 column（250 mm× 4.6 mm,5 μm） was used with a mobile phase consisting of CH_3CN-H_2O（30：70） at the flow rate of1.0 mL/min;the column temperature was maintained at 30 ℃,and the detection wavelength was set at 270 nm.Results The standard curve for icariin was linear in the range of 0.010 2-0.101 5 μg.The average recovery was 99.38%with RSD of 1.51%（n=6）.Conclusion The method is simple,accurate,reproducible,and can be used to the quality control of Baji Oral Liquid.