PRDM16和BMP7基因表达量在中缅树鼩体重调节中的作用

Roles of PRDM16 and BMP7 gene expressions in body mass regulation in Tupaia belangeri

为探讨季节性环境变化下中缅树鼩PRDM16(PR domain-containing 16)和BMP7(bone morphogenetic proteins 7)基因表达量对其生理适应性调节的作用,本研究测定了野外不同季节和实验室冷驯化条件下中缅树鼩的体重、静止代谢率(resting metabolic rate,RMR)、非颤抖性产热(nonshivering thermogenesis,NST)、摄食量、PRDM16和BMP7基因表达量的变化。结果表明:季节性变化过程中中缅树鼩的体重、RMR、NST、褐色脂肪组织(brown adipose tissue,BAT)重、白色脂肪组织(white adipose tissue,WAT)重和摄食量均是冬季显著高于夏季;中缅树鼩不同季节的PRDM16和BMP7基因表达量差异极显著,PRDM16表达量的季节变化趋势为:冬季>秋季>春季>夏季;BMP7表达量的季节变化趋势为:冬季>秋季>夏季>春季。冷驯化条件下,中缅树鼩的体重、RMR、NST、摄食量、BAT含量、大网膜WAT含量显著增加,PRDM16和BMP7基因表达量也显著增加。以上结果表明,中缅树鼩褐色脂肪细胞存在PRDM16和BMP7肌源性起源,即冬季或者低温条件下中缅树鼩PRDM16和BMP7表达量上调,促进褐色脂肪细胞形成,增加NST来弥补产热的不足,以适应冬季寒冷的环境。PRDM16和BMP7在中缅树鼩季节性产热调节和能量代谢中起着重要的作用。

In order to clarify the adjustment roles of PR domain-containing 16 （PRDM16） and bone morphogenetic proteins 7 （BMP7） genes expressions in the physiological adaptation of tree shrew （Tupaia belangeri） to seasonal environment changes, the indexes of tree shrew＇ s body mass, resting metabolic rate （RMR）, nonshivering thermogenesis （NST）, food intake, brown adipose tissue （BAT） mass, white adipose tissue （WAT） mass, PRDM16 and BMP7 genes expressions were measured under different seasons and cold acclimatization. The results showed that, with the seasonal changes, tree shrew＇ s body mass, BAT mass, WAT mass, RMR, NST and food intake in winter were significantly higher than that in summer; its PRDM16 and BMP7 genes expressions showed significant differences in different seasons. Seasonal variation tendency of PRDM16 gene expression was in order of winter〉autumn〉spring〉 summer, and the seasonalvariation tendency of BMP7 gene expression was in order of winter〉autumn〉summer〉spring. Cold acclimatization increased its body mass, BAT mass, WAT mass, RMR, NST and food intake. PRDM16 and BMP7 genes expressions were also increased under cold acclimation. The results suggested that the gene expression levels of PRDM16 and BMP7 of the tree shrews were increased in winter or with cold exposure, which promoted the formation of brown fat cells to increase the thermogenesis to adapt to the cold condition in winter. Moreover, PRDM16 and BMP7 genes play important roles in the seasonal thermogenie regulation and energy metabolism in the tree shrews.