猕猴桃^60Co-γ射线辐射诱变植株变异的ISSR分子标记研究

Study on ISSR Molecular Marker of Kiwifruit Mutational Plant Induced by ^(60)Co-γ Ray

分析不同辐射处理后"长果"猕猴桃(Actinidia longicarpa)样本的变异性,为猕猴桃诱变育种及加快猕猴桃育种进程提供理论基础。采用0(CK)、40、60、80和100 Gy^(60)Co-γ射线辐射处理"长果"猕猴桃接穗后,用ISSR分子标记技术对5种处理后的55个样品的多态性进行研究。结果表明:从100条寡聚核苷酸引物中筛选出7条引物进行标记,平均每条引物扩增出14.3条带,其中6.7条多态性带,多态性比率为46.85%;遗传相似系数为0.79~1.00,聚类分析表明,第V类为60Gy 6~8号植株,与对照的遗传距离最远,变异程度最大。因此,辐射诱变可导致猕猴桃发生显著的遗传变异,利用ISSR分析方法,可准确测定辐照样本是否为突变体。

In order to provide theoretical base for kiwifruit mutation breeding and speed up the breeding process, the variability among irradiation-treated kiwifruit ＇ Changguo＇ samples were analyzed. The branches of kiwifruit ＇ Changguo＇ from 55 samples were irradiated by ^60Co-γ ray in 0 （CK） , 40, 60, 80 and 100 Gy, respectively. The polymorphism of these 5 treatments were analyzed using ISSR molecular marker technology. The results showed that 7 screened primers were used for marking from 100 oligonucleotide primers. The average amplified fragment was 14.3, and the polymorphic bands number was 6.7. The polymorphic ratio was 46.85 %. Calculated genetic similarity coefficient was from 0.79 to 1.00. Clustering analysis suggested that the V class was 6 - 8 plants of 60Gy, which the genetic distance was the farthest, and the variation was the largest compared with others. Large genetic variation in kiwifruit was induced by irradiation. It is accurate to measure whether irradiation samples are mutants using ISSR for mutant cultivar.