含辅酶Q_(10)的红曲胶囊对去卵巢合并D-半乳糖大鼠股骨的影响

Protective effect of red yeast rice capsule containing coenzyme Q_(10) on osteoporosis in rats induced by ovariectomy combined with D-galactose

目的采用大鼠去卵巢合并D-半乳糖诱导老年性骨质疏松的动物模型来评价含辅酶Q_(10)的红曲胶囊对其股骨的骨药理学作用,并与雌激素己烯雌酚比较。方法 3 mon龄SPF级♀SD大鼠,随机分为假手术对照组(CON),去卵巢组(OVX)、模型组(MOD),己烯雌酚组(DES)和红曲胶囊组(RYR),连续给药60 d。实验结束时,取左侧股骨检测钙、磷和羟脯氨酸含量;取右侧股骨进行三点弯曲实验,然后用Micro CT进行骨量分析。结果与CON组相比,MOD组的大鼠体重明显增加;股骨的最大载荷、刚度、最大强度、断裂强度、弹性模量均明显降低;股骨的Ca、P、Hyp含量均明显降低;Micro CT指标BV/TV、Tb.N、BMD、Conn-Dens均明显降低,Tb.Sp、SMI均明显增加。与MOD组相比,RYR组体重明显降低;各力学指标均有增加的趋势;Ca和P的含量均明显增加;BV/TV、Tb.N、Tb.Th、BMD、Conn-Dens均明显增加,Tb.Sp明显降低。与MOD组相比,DES组股骨的各项指标趋势与RYR组相一致。但与DES组相比,RYR组体重明显降低,Ca和P含量明显增加,BV/TV、Tb.N、Tb.Th、ConnDens明显升高,Tb.Sp、SMI明显降低。结论去卵巢合并D-半乳糖可诱导大鼠股骨出现老年性骨质疏松的表现,含辅酶Q_(10)的红曲胶囊可使去卵巢合并D-半乳糖大鼠的体重明显减轻,股骨矿物质含量丢失得到恢复;股骨的骨丢失明显减轻,骨微观结构得到明显改善。且0.5颗·kg^(-1)的红曲胶囊在增加松质骨骨小梁数量上比30μg·kg^(-1)己烯雌酚效果要好。

Aim To investigate the effects of red yeast rice capsules containing coenzyme Q_（10） on femur with an animal model of osteoporosis,which was induced by OVX with D-galactose in rats,and the results were compared with those obtained from diethylstilbestrol.Methods Three-month old female Sprague-Dawley rats were randomly divided into sham group（ CON）,ovariectomized group（ OVX）,model group（ MOD）,diethylstilbestrol group（ DES）,and red yeast rice capsule group（ RYR）. After 60 days,the left femurs were collected for Ca,P and hyp measurement,while the right femurs were performed with three-point bending test and micro-CT evaluation,respectively. Results Compared with CON group,MOD group had a significant increase in body weight,Tb. Sp,SMI and significant decrease in maximum load,stiffness,maximum strength,break strength,elastic modulus,Ca,P,Hyp contents and indicators of BV / TV, Tb. N, BMD,Conn-Dens. On the other hand,compared with MOD group,RYR group had a lower body weight and all bone biomechanics indexes were increased without statistically significant difference. At the same time,the content of Ca,P and indicators of BV / TV,Tb. N,Tb.Th,BMD,Conn-Dens increased significantly; yet Tb.Sp decreased significantly. In DES group,the results of indicators were consistent with those for RYR group. In addition,compared with DES group,in RYR group body weight decreased significantly; the content of Ca,P and indicators of BV / TV,Tb. N,Tb. Th,ConnDens were significantly higher,and Tb. Sp,SMI were significantly lower. Conclusions Significant bone loss and deteriorated mechanical properties of femur can be observed in animal model of osteoporosis induced by OVX combined with D-galactose. Red yeast rice capsules containing coenzyme Q_（10） show effective prevention effects. Furthermore,red yeast rice capsules（ 0. 5 tablet·kg^（- 1）） have better effect on increasing the number of trabecular bone than diethylstilbestrol（ 30 μg ·kg^（- 1）） does.