异可利定对大鼠心肌缺血及缺血/再灌注所致心律失常的影响

Effect of isocorydine on arrhythmia in rats induced by myocardial ischemia / reperfusion injury

目的探究异可利定对大鼠心肌缺血及缺血/再灌注所致心律失常的影响。方法将SD大鼠随机分为假手术组、心肌缺血/再灌注模型组、阳性药维拉帕米组、异可利定(2.5、5、10 mg·kg^(-1))组,共6组,每组16只。结扎大鼠左冠状动脉建立大鼠心肌缺血/再灌注损伤模型,记录Ⅱ导联心电图,并测定大鼠心肌缺血面积、心肌梗死范围及心肌组织超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)等生化指标。结果异可利定可以明显降低大鼠缺血/再灌注室性早搏(ventricular extrasystole,VE)、室性心动过速(ventricular tachycardia,VT)、心室颤动(ventricular fibrillation,VF)的发生率;减少缺血期间大鼠心肌缺血面积和心肌梗死范围;明显升高缺血/再灌注损伤心肌SOD、GSH-Px的含量,降低MDA含量。结论异可利定对心脏缺血/再灌注损伤有保护作用,可能与其抗氧化作用有关。

Aim To investigate the effect of isocorydine on arrhythmia in rats induced by myocardial ischemia /reperfusion injury. Methods SD rats were randomly divided into 6 groups： sham group,myocardial ischemia / reperfusion group, verapamil group and isocorydine group of 2. 5,5,10 mg · kg^（- 1）,each group having 16 rats. Left anterior descending（ LAD） was tied up to establish the injury model of myocardial ischemia. ECG was recorded for analysis. The ischemic and infarction area was measured and indexes of SOD,MDA,GSH-Px in the myocardial were determined. Results Isocorydine could significantly reduce the incidence of arrhythmia induced by ischemic / reperfusion,including VT,VF; and reduce ischemic and infarction area. Further study demonstrated that isocorydine could increase myocardial SOD and GSH-Px,but decrease myocardial MDA. Conclusion Isocorydine has a protective effect on the myocardial ischemia / reperfusion injury,which might be related to its anti-oxidative function.