3种小鼠晶状体组织石蜡切片固定液的比较

Comparison of Fixation Effects of Three Different Complex Fixatives on Mouse Lens Tissue

目的比较3种固定液对小鼠晶状体组织石蜡切片的影响,优化小鼠晶状体组织石蜡切片固定方法。方法选取3种常用固定液(改良Davison’s固定液、传统Davison’s固定液和10%中性甲醛固定液),固定小鼠晶状体组织,并制成石蜡切片,经HE染色后在显微镜下观察。结果传统Davison’s固定液固定的晶状体组织切片无碎裂,改良Davison’s固定液固定的晶状体组织中央区碎裂。改良Davison’s固定液和传统Davison’s固定液固定的小鼠眼球外形均无明显变形和收缩,未见明显的视网膜脱离,镜下视网膜组织结构完整清晰,各层细胞排列紧密,无明显裂隙,染色鲜艳。采用10%中性甲醛固定液固定的小鼠眼球壁组织发生严重皱缩,晶状体组织皱缩,空隙和空泡形成。结论传统Davison’s固定液固定的小鼠眼球晶状体组织石蜡切片质量效果优于改良Davison’s固定液和10%中性甲醛固定液。

Objective To compare the effects of three kinds of fixative solutions on paraffin section of mouse lens tissue and optimize the fixingmethod of paraffin section in mouse lens tissue. Methods Three kinds of conventional fixatives were selected for the test, including the conventional Davison＇ s solution, modified Davison＇ s solution and 10% neutral buffered formalin. Mice eyeball tissues were fixed with three different fixatives, embedded, shced and then stained with HE method. The paraffin slices were observed under the light microscope. Results The structures of lens and retina fixed in conventional Davison＇ s fixative solutions were clear and intact, and the cells were arranged regularly and compactly. There was no eyeball distortion, contraction and retinal detachment in the eyeballs fixed in modified and conventional Davison ＇ s fixative solution. However, the ones fixed in 10% neutral buffered formalin showed eyeball distortion and contraction, space and sphemles. Condusion The mice lens slides made from tissues fixed by conventional Davison ＇ s fixative solution me better than fixed by modified Davison ＇ s fixative solution and the 10% neutral buffered formalin fixed ones.