β-七叶皂甙钠对脊髓损伤大鼠脊髓组织水通道蛋白4与水通道蛋白9表达的影响

Effect of β-sodium Aescinate on the Expression of Aquaporin-4 and Aquaporin-9 in Rats with Spinal Cord Injury

目的探讨β-七叶皂甙钠对脊髓损伤大鼠水通道蛋白(AQP)4与AQP9表达的影响。方法成年雌性SD大鼠150只,随机分为假手术组、脊髓损伤组和β-七叶皂甙钠组,每组50只。用改良的Allen’s法建立急性脊髓损伤模型后,术后行BBB运动功能评分及斜板实验评价大鼠后肢功能,免疫组化和Western blotting方法检测各组大鼠脊髓组织AQP4与AQP9的表达变化。结果相比于假手术组,各时间点脊髓损伤组和β-七叶皂甙钠组运动功能评分及斜板实验评分均显著降低(P<0.05);但是第7天开始,各时间点β-七叶皂甙钠组大鼠后肢功能恢复情况明显优于脊髓损伤组(P<0.05)。相比于假手术组,各时间点脊髓损伤组和β-七叶皂甙钠组大鼠脊髓组织AQP4与AQP9的含量显著升高(P<0.05);但是与同时间点脊髓损伤组相比,β-七叶皂甙钠组大鼠脊髓组织AQP4与AQP9的含量显著降低(P<0.05)。结论β-七叶皂甙钠能够通过降低AQP4与AQP9蛋白表达对脊髓损伤大鼠的神经功能发挥保护作用。

Objective To study the effect of β-sodium aescinate on the expressions of aquaporin-4 and aquaporin-9 in rats with spinal cord injury. Methods A total of 150 rats were randomly divided into 3 groups ： sham group （n = 50）, spinal cord injury（SCI） group （n = 50） and β-sodi- um aescinate group （n = 50）. The experimental animal models was established by modified Allen＇ s model. The Basso, Beattie and Bresnahan （BBB） locomotor rating scale and inclined plane test were used to evaluate rat behavioral consequences after injury.The immunohistochemical staining and western blotting assay were performed to observe the expressions of aquaporin-4 and aquaporin-9. Results Compared with sham group, BBB score and inclined plane test score of SCI group and β-sodimn aescinate group were significantly lower at each time point （P 〈 0.05 ） ; however, the functional recovery was significantly better in β-sodium aescinate group than in SCI group at each time point from 7 d after SCI （P 〈 0.05 ）. The aquaporin-4 and aquaporin-9 positive expressions of rats in sham group were lower significantly than rats in SCI group and β-sodium aescinate group （P 〈 0.05 ） ;however, the aquaporin-4 and aquaporin-9 positive expressions of rats inβ-sodium aescinate group was lower significantly than rats in SCI group at each time point （P 〈 0.05 ）. Conclusion β-sodium aescinate can protect the neurologic function in rats with spinal cord injury by decreasing aquaporin-4 and aquaporin-9 protein expression.