细锯脂鲤背鳍和脂鳍的发育观察和转录组比较分析

Oberservation and transcriptomic analysis of the development of dorsal fin and adipose fin in Pristella maxillaris

观察了细锯脂鲤脂鳍和背鳍形态和骨骼发育,在此基础上比较了脂鳍、背鳍原基和背鳍共3个组织的转录组,发现细锯脂鲤背鳍原基28 DAH(Day After Hatch,DAH)出现,30 DAH仔鱼背鳍鳍条已出现分化,35 DAH背鳍鳍条已硬骨化,脂鳍在32 DAH开始出现。背鳍原基、背鳍和脂鳍这3个组织共同表达基因有14 115条,分别占背鳍原基转录组的84.01%、背鳍的83.19%、脂鳍的84.14%,提示脂鳍发育的主要调控机制与背鳍发育较为相似。相同基因表达水平差异方面,相比脂鳍,背鳍原基和背鳍之间的基因表达模式要更为相似。发现有些与鳍发育相关的基因在背鳍和脂鳍转录组中存在差异。这些研究结果为进一步深入了解脂鳍发育奠定了基础。

So far we have no clear understanding on the adipose fin development.In order to find out the differences between the adipose fin and dorsal fin developments,we investigated the developing process and ossification process of dorsal fin and adipose fin in Pristella maxillaris.We found that the dorsal fin bud showed up from the larva fish dorsal at 28 DAH（ day after hatch）,dorsal fin ray could be seen at 30 DAH,and dorsal fin rays ossified at 35 DAH,whereas,the adipose fin could be found from 32 DAH.Based on this observation,we sequenced the transcriptoms of the dorsal fin bud at 28 DAH,dorsal fin at 30 DAH and adipose fin at 35 DAH in P.maxillaris.A series of bioinformatic analysis showed that all three tissues had14 115 same genes,accounting for 84.01% of the dorsal fin bud＇s transcriptome,83.19% of the dorsal fin＇s transcriptome,and 84.14% of the adipose fin ＇s transcriptome,indicating that there might be similar molecular mechanism of the development process of adipose fin and dorsal fin in P.maxillaris.At gene expression level,compared with adipose fin,dorsal fin bud and dorsal fin expression was more similar.Furthermore,we found that some genes relative to the fin development such as tbx5,bmp4 etc,were expressed differently in adipose fin and dorsal fin.These information provided by the comparative transcriptomic analysis may help us to understand the development of adipose fin betterly.