土贝母皂苷抑制对苯二胺诱发豚鼠接触性皮炎效应的研究

Studies on inhibitory effect of tubeimosides on delayed contact hypersensitivity induced by p-phenylenediamine in guinea pigs

目的:研究土贝母皂苷(Tubeimosides,TBMSs,简称皂苷)抑制对苯二胺诱发的豚鼠接触性皮炎的效应。方法:从土贝母块茎中提取分离出有效部位土贝母皂苷。实验所用皂苷的主要成分是土贝母苷甲和苷乙(简称苷甲、苷乙),以及微量土贝母苷丙(简称苷丙),其标志性成分苷甲的含量≥90%。采用异体和同体两种对照的局部涂皮封闭实验(Buehler test),研究皂苷对对苯二胺(p-phenylenediamine,PPDA)诱发豚鼠接触性皮炎的效应的影响。结果:异体和同体对照实验结果表明,皂苷提前或与PPDA混合应用,均可剂量依赖性地改善,甚至完全抑制PPDA诱发豚鼠接触性皮炎。一分子的土贝母皂苷能抑制或完全逆转6到12分子对苯二胺诱发的豚鼠接触性皮炎。结论:土贝母皂苷有显著抑制对苯二胺诱发豚鼠接触性皮炎的活性。

Objective： To study the inhibitory effect of tubeimosides on the delayed contact hypersensitivity induced by p-phenylenediamine in guinea pigs. Methods： Tubeimosides（ TBMSs） was the effective fraction extracted and isolated from the tubers of Bolbostemma paniculatum. The main ingredients of TBMSs used in the experiments were tubeimoside Ⅰ,tubeimoside Ⅱ and a trace of tubeimoside Ⅲ,wherein the content of tubeimoside Ⅰ was equal to or greater than 90%. The closed or occlusive patch tests（ Buehler test） of heterogeneous and autogenous contrast on guinea pigs were utilized to this investigation. Results： TBMSs could significantly improve,or even completely suppressed p-phenylenediamine（ PPDA）-induced contact dermatitis in guinea pigs in a dose-dependent manner,and that 1 molecule of TBMSs could inhibit or completely reverse the delayed contact hypersensitivity induced by approximately 6 to 12 molecules of PPDA. Conclusion： TBMSs have obvious activity against the delayed contact hypersensitivity induced by PPDA in guinea pigs.