KLF9和KLF15在维吾尔族网膜脂肪中的表达及其与炎症相关性分析

KLF9,KLF15 expression in omental adipose tissue and correlation with inflammatory of an Uygur population

目的:检测维吾尔族网膜脂肪组织KLF9及KLF15 m RNA表达水平,分析KLF9、KLF15与炎症信号通路关键因子的相关性。方法:选取维吾尔族个体NC组50例、OB组45例,检测一般资料和生化指标。采集网膜脂肪组织,RT-PCR法检测KLF9、KLF15及NF-κB炎症信号通路关键因子m RNA表达水平。结果:OB组BMI、WC、HC、WHR、TG和LDL显著高于NC组,差异有统计学意义(P<0.05)。维吾尔族网膜脂肪组织中,OB组NF-κB、TNF-α和IL-6 m RNA表达水平显著高于NC组(P<0.05),NF-κB m RNA表达与体重和TG显著正相关(P<0.05),TNF-αm RNA表达与TG和TC显著正相关(P<0.05),IL-6 m RNA表达与HC、TG和LDL显著正相关(P<0.05);OB组网膜脂肪组织KLF15 m RNA表达水平显著低于NC组(P<0.05),与BMI、WC、WHR显著负相关(P<0.05),与HDL正相关,与TG负相关。KLF9 m RNA表达水平高于NC组,与NF-κB显著正相关(P<0.01),与IL-6正相关。结论:KLF15可能通过调控脂代谢发挥抗炎作用;肥胖状态下脂代谢紊乱可能间接上调KLF9的表达,而KLF9作为转录激活因子可能通过调控下游炎症因子的转录活性,从而促进炎症因子的表达和释放。

Objective： To detect KLF9 and KLF15 m RNA expression of the omental adipose tissue,and analyze the relationship between KLF9,KLF15 and inflammatory in an Uygur population. Methods： The Uygur subjects were selected and divided into NC group（ n = 50） and OB group（ n = 45）. The m RNA level of KLF9,KLF15 and the critical factors of NF-κB inflammatory signaling pathways were detected by RT-PCR. Results： Compared with NC group,the level of BMI,WC,HC,WHR,TG and LDL were significantly higher in the OB group（ P0. 05）; the m RNA level of NF-κB,TNF-α and IL-6 were significantly higher in the OB group（ P 0. 05）; NF-κB m RNA expression level was significantly positive correlated with weight and TG（ P 0. 05）; TNF-α m RNA expression level was significantly correlated with TG and TC; IL-6 m RNA expression level was significantly correlated with HC,TG and LDL（ P0. 05）. The KLF15 m RNA expression level of the omental adipose tissue in OB group was significantly higher than the NC group（ P0. 05）; and significantly positive correlated with BMI,WC and WHR（ P0. 05）,positive correlated with HDL,while negative correlated with TG. Conclusion： KLF15 probably plays the important role of anti-inflammatory by regulating lipid metabolism; under the obesity condition,Lipid metabolism disorders may indirectly increase KLF9 expression,and KLF9 may be act as an activating transcription factor to promote the expression and release of inflammatory cytokines through regulating the transcription activity of inflammatory factors downstream.