苹果轮纹病菌抗戊唑醇突变体UV-TS1-10的生理生化特性

Physiological and biochemical characteristics of tebuconazole-resistant isolate of Botryosphaeria dothidea UV-TS1-10

利用苹果轮纹病菌敏感菌株TS1和抗戊唑醇突变体UV-TS1-10进行了苯丙氨酸解氨酶（PAL）、过氧化物酶（POD）活力、可溶性蛋白含量及酯酶同工酶图谱等生理生化特性的测定。经不同浓度药剂处理0~24h,抗性突变体UV-TS1-10体内PAL活性一直高于敏感菌株TS1,两者都呈现先上升后下降的趋势,并在1.5h达到最高值;抗药突变体UV-TS1-10体内POD活力也高于TS1,一直处于上升趋势,24h达到最高值时两菌株活力差异最大;UV-TS1-10体内可溶性蛋白含量为TS1的1.3倍,同一菌株在不同浓度戊唑醇处理后可溶性蛋白含量差异不大,稳定性好;抗药突变体UV-TS1-10比敏感菌株TS1的酯酶同工酶图谱少了一条Rf=0.33的谱带,多了Rf=0.14的特征性谱带,表明抗戊唑醇突变体UV-TS1-10其生理生化特性发生了较大变化。本文以研究敏、抗菌株间生理生化特性的差异为基础,探讨苹果轮纹病菌对戊唑醇可能的抗性机制,为科学地指导生产用药提供理论依据。

A tebuconazole-resistant isolate of Botryosphaeria dothidea,UV-TS1-10,was isolated by UV treatment of sensitive strain TS1,and its physiological and biochemical characteristics of phenylalanine ammonia lyase（PAL）,peroxidase（POD）,soluble protein contents and electrophoretic patterns of esterases were determined.The results showed that,when treated with different concentrations of tebuconazole for 0-24 h,the activity of PAL of UV-TS1-10 was higher than that of TS1 at all time.The value of PAL was increased firstly and peaked at1.5 h,and then decreased.The values of POD in the two isolates were rising all the time,and reached the top at24 h,and the difference between the two isolates reached the top at that time.The soluble protein content of UVTS1-10 was 0.3 folds higher than that of TS1 and there was no difference for the same strain with different concentrations of tebuconazole.In the electrophoretic patterns of esterases,one characteristic band（Rf=0.33）was lost and a new one（Rf=0.14）was found in UV-TS1-10.As the result showed,the development of fungicide resistance was accompanied by the changes of the physiological and biochemical characteristics.The study of sensitive and resistant strains of B.dothidea has great significance for formulating strategies of resistance management and monitoring resistance groups of B.dothideain the field for the future.