北京平谷区苹果锈果类病毒检测初报

Detection of Applescarskinviroidin apple leaves from Pinggu District in Beijing

采集北京市平谷区‘中秋王’、‘寒富’苹果叶片样品共12份,以Northern杂交、RT-PCR方法检测苹果锈果类病毒（Apple scar skin viroid,ASSVd）带毒情况。结果表明,表现明显锈果症状的‘中秋王’样品均检出ASSVd,无明显症状的‘中秋王’样品没有检出ASSVd,而无明显症状的‘寒富’样品全部携带ASSVd。测序发现,所得序列与NCBI中已报道的ASSVd基因组序列相似性为91%～99%,且两品种中ASSVd主流序列完全相同。结果说明‘中秋王’对ASSVd较为敏感,感病后易于表现出明显的锈果症状;‘寒富’对ASSVd耐性较强,感病后不表现出明显症状。

Twelve‘Zhongqiuwang＇and‘Hanfu＇apple leaf samples were collected from Pinggu District,Beijing,China,and Apple scar skin viroid（ASSVd）was detected in these samples using Northern blot and RT-PCR.The results showed that symptomatic‘Zhongqiuwang＇and asymptomatic‘Hanfu＇samples were infected by ASSVd,while no ASSVd was detected in asymptomatic‘Zhongqiuwang＇samples.Sequencing analysis showed that the obtained ASSVd sequences had 91%-99%similarities with other ASSVd sequences in the NCBI,and the dominant sequences of ASSVd from ‘Zhongqiuwang＇and‘Hanfu＇were the same.Our results demonstrated that‘Zhongqiuwang＇seems to be susceptible and sensitive to ASSVd,and‘Hanfu＇showed a relatively strong tolerance to ASSVd.