柳杉种子合子胚的愈伤组织诱导及植株再生

Callus induction and plant regeneration from zygotic embryos of Cryptomeria fortunei

为探索柳杉种子合子胚的愈伤组织诱导技术,选取柳杉4个家系,采集其尚未完全成熟的种子进行愈伤组织的诱导,并经器官发生再生途径得到再生植株。利用6-BA、2,4-D等激素种类及其浓度的正交设计,研究各因素及其交互作用对柳杉种子合子胚愈伤组织诱导的影响。结果发现:柳杉4个家系的愈伤组织诱导量在8月中旬采种时呈明显上升,至8月底时各家系的子叶胚愈伤诱导率达到最高,其中57号家系愈伤诱导率最高,达92%。柳杉4个家系,在不同激素组合中愈伤诱导率存在着一定的差异。其中57号家系子叶胚在DCR+6-BA1.0 mg/L+2,4-D 1.0 mg/L培养时,愈伤组织诱导率达到最高,且诱导出来的愈伤组织状态为白色、干燥、无针状突起,结构紧密。愈伤组织在DCR培养基上成功获得再生植株。

In this article,immature seeds from 4 families were selected to conduct callus induction and then regeneration plants were obtained by organogenesis in Cryptomeria fortunei by orthogonal design with different types of hormones and concentration. The effect caused by respective factors and interactions on callus induction from zygotic embryos of Cryptomeria fortunei were studied. The following were the results ： （1） The amount of callus induction from 4 families increased obviously collecting seeds in the mid August. By the late August, the callus induction rate from each zygotic embryos reached the highest, among which family NO. 57 family took the first place （92%）. （2） There were certain differences between the induction rate by using different hormone combinations. The highest induction rate of callus was that of family NO. 57 family which grew on DCR＋6-BA 1.0 mg/L ＋2,4-D 1.0 mg/L culture medium,and the callus induced was White, dry and of highly dense form, with no needle. （3） Regeneration plants were successfully abtained from callus on the DCR basic medium.