荷叶离褶伞发酵液对NaCl胁迫下洋葱根尖细胞有丝分裂的影响

Effects of Fermentative Fluid of Lyophyllum decastes on Mitosis of Root Tip Cells of Allium cepa L. Under NaCl Stress

研究了荷叶离褶伞发酵液对NaCl胁迫下洋葱根尖细胞有丝分裂的影响。结果表明:低浓度NaCl(0.05 mol/L)对洋葱根的生长有促进作用,高浓度NaCl(≥0.10 mol/L)对洋葱根的生长有抑制作用,根尖细胞有丝分裂出现异常,且随着NaCl浓度增高和NaCl处理时间延长抑制作用越明显。荷叶离褶伞发酵液对低浓度NaCl(≤0.10 mol/L)短时间处理(24 h)导致的洋葱根尖细胞遗传损伤有一定的修复作用。

The root tip of Allium cepa L is treated with different concentrations of NaCl for 24 hours and 48 hours. The results shows that low concentrations of NaCl significantly promoted the growth of Allium cepa L, but higher concentrations（≥0.1 mol/L） played a poisoned role on the growth of Allium cepa L and have a dose-effect relationship. while we cultured the different NaCl treated Allium cepa L using diluted Fermentative Fluid of Lyophyllum decastes for 24 hours, the result shows that diluted Fermentative Fluid of Lyophyllum decastes can repair the genetic damage and promote the growth of a short period of time NaCl treated Allium cepa L （24 h）, such as decreased micronucleus rate and chromosome aberration rate, increased mitotic index, but have a weak role for a long period of time treated NaCl Allium cepa L（48 h）.