HPLC法测定乌龙茶中6种儿茶素类成分含量

Simultaneous determination of six catechins in Oolong by HPLC

采用HPLC外标法测定乌龙茶中没食子儿茶素（EGC）、儿茶素（C）、表没食子基儿茶素没食子酸酯（EGCG）、表儿茶素（EC）、没食子基儿茶素没食子酸酯（GCG）、没食子酰表儿茶素（ECG）等6种儿茶素类化合物的含量。选用Shimadzu Wonda Cract ODS-2色谱柱（4.6mm×250mm,5μm）,流动相为0.5%乙酸水溶液（A相）、乙腈（B相）,梯度洗脱：0~40min从10%B线性上升至30%B;流速为1.0mL·min-1,检测波长为280nm,柱温为35℃。结果表明5种不同品牌的乌龙茶中EGC、C、EC、EGCG、GCG和ECG的含量分别为19.5~85.3mg/g、152.9~376.5mg/g、4.7~19.2mg/g、39.4~181.9mg/g、10.0~74.3mg/g和15.4~64.2mg/g,本方法可以准确地测定乌龙茶中的6个指标成分的含量,为乌龙茶质量评价提供依据。

HPLC separation was performed on a Shimadzu Wonda Cract ODS-2analytical column（250mm×4.6mm,5μm）.with a flow rate of 1.0mL·min-1.while the column temerature was 35 ℃.The mobile phase consisted of water containing 0.5%acitic acid（A）and acetonitrile（B）with a linear gradient elution of 10%-30% B for 40 min.The detection wavelength was 280 nm.The contents of EGC,C,EC,EGCG,GCG and ECG were 19.5-85.3mg/g,152.9-376.5mg/g,4.7-19.2mg/g,39.4-181.9mg/g,10.0-74.3mg/g and 15.4-64.2mg/g,respectively.