分子生物学方法鉴定新资源食品中乳杆菌

Identification of Lactobacillus of New Resource Food by Molecular Biology Methods

采用16S rDNA扩增同源性分析、16S rDNA PCR-RFLP技术、种特异性序列扩增技术,对新资源食品中的副干酪乳杆菌Lactobacillus paracasei、鼠李糖乳杆菌Lactobacillus rhamnosus、嗜酸乳杆菌Lactobacillus acidophilus、植物乳杆菌Lactobacillus plantarum进行鉴定分析。结果表明,16S rDNA扩增同源性分析能够有效鉴定嗜酸乳杆菌、植物乳杆菌,无法区分副干酪乳杆菌和鼠李糖乳杆菌;16S rDNA PCR-RFLP技术可以有效鉴定嗜酸乳杆菌、植物乳杆菌、副干酪乳杆菌和鼠李糖乳杆菌;种特异性序列扩增能够有效鉴定副干酪乳杆菌和鼠李糖乳杆菌。结合检验工作需要,运用以上方法可以对食品安全国家标准中生化鉴定法难以确认的乳杆菌进行有效鉴别。

Identification of four species（L.paracasei,L.rhamnosus,L.plantarum,L.acidophilus） of Lactobacillus of new resource food by using techniques of the analysis of 16 S r DNA homology amplification,16 S r DNA PCR-RFLP and species-specific sequence amplification. The results show that the analysis of 16 S r DNA homology amplification can identify L.plantarum and L.acidophilus effectively,but it is not useful to distinguish between L.paracasei and L.rhamnosus. 16 S r DNA PCR-RFLP technique identify L.paracasei,L.rhamnosus,L.plantarum,L.acidophilus effectively. Species-specific amplification can effectively identify L.paracasei and L.rhamnosus. Considering the requirements of examination,the above methods can be used to identify the strains which can not be identified by the biochemical identification method described in the national standard of food safety.