摘要
通过时GenBank中马鼻疽伯克霍尔德氏菌(Burkholderia mallei)、亨德拉病毒(Hendra virus,Hev)和西尼罗热病毒(West nile virus,WNV)的主要基因进行分析比较,分别设计合成了引物和TaqMan荧光探针,对反应条件和试剂浓度进行优化,建立了能同时检测这3种病原的三重实时荧光定量PCR方法。本研究建立的三重荧光定量PCR具有良好的特异性、敏感性和重复性,适合于大批量样品的检测,可广泛用于出入境马匹这3种病原体的检疫。
A multiplex real-time PCR was developed using the primers and probes designed according to the highly conserved regions of genes of Burkholderia mallei,Hendra virus and West Nile virus reported in GenBank.The results showed that this assay was sensitive,specific and reproducible for detection of entry and exit equine samples.
出处
《中国动物传染病学报》
CAS
北大核心
2016年第4期36-40,共5页
Chinese Journal of Animal Infectious Diseases
基金
上海检验检疫局科技计划项目(HK002-2015)
