4-氯苯酚对斑马鱼胚胎急性发育毒性与细胞凋亡的研究

Acute Developmental Toxicity and Apoptosis in Zebrafish Embryos Treated by 4-chlorophenol

实验以斑马鱼胚胎为研究对象,考察不同浓度的4-氯苯酚(1 mg/L、5 mg/L、10 mg/L)对斑马鱼胚胎的急性毒性及细胞凋亡.分别在24 h、48 h、72 h、96 h、120 h时测定胚胎的急性发育指标及细胞凋亡.结果显示,在不同浓度的4-氯苯酚溶液中,斑马鱼的孵化率随着暴露剂量的增加而降低,斑马鱼的死亡率与畸形率随着暴露剂量的增加而升高,形态学异常的主要表现为心包囊肿、脊柱弯曲、卵黄囊畸形等多种毒性效应;同时AO染色法以及细胞凋亡酶的结果显示4-氯苯酚能够诱导胚胎产生细胞凋亡.

The study was to investigate the acute toxicity of 4-chlorophenol to zebrafish embryos. The indicators included developmental toxicology and apoptosis were determined at 24 h,48 h,72 h,96 h and 120 h. The results showed as followed： accompanition with the increasing exposures concentrations,the survival rates and hatching rates were decreased,the malformation rates were increased and the hearting rates were accelerated.Moreover,the 4-chlorophenol exposures induced kinds of morphologic abnormalities,such as pericardium edema,spinal curvature and yolk sac edema. At the same time,the results of acridine orange stain and the activity of caspase-9 suggested 4-chlorophenol induced apoptosis to zebrafish embryos.