摘要
以正十六烷无机盐培养基为选择培养基,从武汉石化输油管附近土壤中筛选出了1株高效降解长链烷烃的菌株C3。采用摇瓶实验,研究了菌株C3降解正十六烷的降解条件、降解动力学及降解机理。菌株C3降解正十六烷的最适宜条件为温度35℃、初始p H=7、摇床转速150 r/min。动力学研究显示,在底物质量浓度为1 000 mg/L时,其降解动力学拟合结果符合Monod方程,半饱和常数Ks=609.8 mg/L,最大降解速率v_(max)=62.1mg/(L·h)。对C3降解正十六烷产物进行红外光谱及GC/MS分析,推测该降解方式为末端氧化。
Regarding the mineral salt medium with the n- hexadecane as the selecting medium,strain C3 that can effectively degrade the long- chain alkane is screened out from the soil near the pipeline of Wuhan Petrochemical Company. The conditions of degrading n- hexadecane,growth kinetics and mechanism of degradation are studied by shaking flask tests. It is found that the optimum conditions for the growth of strain C3 are under 35℃,pH = 7 and 150 r/min of table speed. The growth kinetics research shows that when the concentration of n- hexadecane is 1 000 mg/L under optimum conditions,the n- hexadecane degradation is in accordance with Monod equation,with Ksbeing 609. 8 mg/L and vmaxbeing 62. 1 mg/（L·h）. The products from the degradation are analyzed by infrared spectrometer and GC/MS and it is forecast that the degradation is terminal oxidation.
出处
《工业安全与环保》
北大核心
2016年第11期64-67,共4页
Industrial Safety and Environmental Protection
关键词
长链烷烃
降解条件
MONOD方程
末端氧化
long-chain alkane
degradation conditions
Monod equation
terminal oxidation
