10个山东主栽石榴品种果实褐变及其相关酶活性比较

Fruit Browning and Related Enzyme Activity Comparison of 10 Dominant Pomegranate Cultivars in Shandong

以10个山东主栽石榴品种为试材,比较分析了其成熟期果实品质、褐变及其相关酶活性、总酚含量和抗氧化能力的差异及相关关系。结果表明,不同石榴品种果实各品质指标存在显著差异,‘泰山红’单果重和可溶性固形物含量最高,‘秋艳’百粒重最大,‘大青皮酸’可滴定酸含量最高,‘泰山三白甜’固酸比和可溶性糖含量最高。不同品种果实褐变及其相关酶活性、总酚含量和DPPH自由基清除率存在显著差异。‘泰山三白甜’果实褐变度、PPO、POD、PAL活性、总酚含量和DPPH自由基清除率均最高。果实品质各指标间存在不同的相关性,褐变度与果实品质、相关酶活性、总酚和DPPH自由基清除率间存在一定的相关性。PPO、POD是影响石榴果实褐变的主导酶类,PAL在一定程度上影响其褐变。果实酚类物质含量高低与其抗氧化能力密切相关。

Taking 10 dominant pomegranate cultivars in Shandong as materials,the difference and correlation of fruit quality,browning and related enzyme activity,total phenols content,antioxidant capacity were compared and analyzed at maturation stage. The results indicated that the difference of fruit quality was significant in different pomegranate cultivars. The single fruit weight and soluble solid content of ‘Taishanhong＇were the highest. The 100- grain weight of ‘Qiuyan＇was the biggest. The titratable acid content of ‘Daqingpisuan＇was the highest. The ratio of soluble solid and titratable acid and soluble sugar content of ‘Taishansanbaitian＇were the highest. There were significant differences in the fruit browning and related enzyme activities,total phenols content and DPPH radial scavenging activity among different pomegranate cultivars. The browning degree,activities of PPO,POD,PAL,total phenols content and DPPH radial scavenging activity of‘Taishansanbaitian＇were all the highest. The fruit quality indexes of different pomegranate cultivars existed different correlations. There was certain correlation of browning degree with fruit quality,related enzyme activities,total phenols,DPPH radial scavenging activity. PPO and POD were the dominant enzymes that influenced the fruit browning of pomegranate. PAL influenced the fruit browning of pomegranate to some extent.The antioxidant capacity of pomegranate fruits was closely related to the contents of phenolic compounds.