好氧反硝化细菌LKX-1的分离、鉴定及初步应用研究

Isolation,Identification of A Aerobic Denitrifier LKX-1 and Its Preliminary Application Research

为筛选分离好氧反硝化细菌菌株用于农村生活污水处理,从水稻田中分离高效好氧反硝化细菌菌株,并进行鉴定及除氮效果分析。结果表明,通过菌体、菌落形态特征和生理生化指标并结合16S r DNA序列分析将该菌株鉴定为蒙氏假单胞菌(Pseudomonas monteilii);农村生活污水除氮条件初步研究结果表明,在葡萄糖添加量0.03%、接种量0.00015%、摇床转速120 r/min和发酵处理时间48 h条件下,总氮和亚硝酸盐氮去除率分别达到91.55%和96.33%。

A high efficient aerobic denitrifier was isolated from rice field to dispose the rural domestic sewage,then the strain was identified and its nitrogen removal effect was analyzed. The results showed that the strain was identified as Pseudomonas monteilii through analyzing the cell morphology,colony morphology,physiological and biochemical characteristics combined with 16 S r DNA sequence. The preliminary study on nitrogen removal conditions of rural domestic sewage showed that with the glucose additive amount of 0. 03%,inoculation amount of 0. 00015%,rotation speed of 120 r/min and cultural time of 48 hours,the removal rate of TN and NO-2- N reached 91. 55% and 96. 33% respectively.