成纤维细胞生长因子受体1在大鼠各组织中的表达

Expression of fibroblast growth factor receptor1 in the tissues of rats

目的研究成纤维细胞生长因子受体1(FGFR1)mRNA及蛋白在大鼠各组织中的表达。方法采用实时定量荧光聚合酶链式反应测大鼠脂肪、肝脏、心脏、血管、肾脏、肌肉等组织中FGFR1 mRNA表达;采用酶联免疫吸附法及免疫组织化学法检测各组织中FGFR1蛋白的表达。结果 FGFR1 mRNA和蛋白在大鼠脂肪、肝脏、心脏、血管、肾脏、肌肉中均有表达,主要分布于细胞膜。FGFR1 mRNA在大鼠脂肪、肝脏、心脏、血管、肾脏、肌肉中表达量分别为2.623 0±0.524 9、1.003 0±0.077 2、6.755 0±0.278 4、1.613 0±0.320 1、4.833 0±0.421 5、6.123 0±0.348 7,FGFR1蛋白在以上各组织中的表达量分别为(10.670 0±1.197 0)、(0.356 0±0.105 5)、(0.860 0±0.163 9)、(5.152 0±0.801 4)、(0.186 0±0.046 7)、(2.110 0±0.161 9)μg·g-1,FGFR1 mRNA在各组织中的表达从高至低依次为心脏、肌肉、肾脏、脂肪、血管和肝脏;FGFR1蛋白在各组织中的表达量从高至低依次为脂肪、血管、肌肉、心脏、肝脏、肾脏。FGFR1 mRNA和蛋白在各组织中的表达量比较差异均有统计学意义(P<0.05,P<0.01)。结论 FGFR1在大鼠各主要组织器官中均有表达,主要分布于细胞膜,且在各组织中的表达存在一定差异。

Objective To study the expression of fibroblast growth factor receptorl （FGFR1） mRNA and protein in the tissues of rats. Methods The expression of FGFR1 mRNA in fat, liver, heart, blood vessel, kedney and muscle tissues was measured by real time-polymerase chain reaction;and the expression of FGFR1 protein was determined by enzyme linked im- munosorbent assay and immunohistochemistry. Results FGFR1 mRNA and protein expressed in fat,liver, heart, blood vessel, kedney and muscle tissues of the rats ; and mainly distributed in the cell membrane. The expression of FGFR1 mRNA in fat, liv- er,heart,blood vessel,kedney and muscle tissues was 2. 623 0±0. 524 9,1. 003 0±0.077 2,6.755 0±0.278 4,1. 613 0 ±0. 320 1,4. 833 0 ± 0. 421 5,6. 123 0 ± 0. 348 7 respectively; the expression of FGFR1 protein in above tissues was （10.6700±1.1970）,（0.356 0 ±0. 105 5）,（0.860 ±0. 163 9）,（5. 152 0±0.801 4）,（0.186 0±0.046 7）, （2.110 0 ±0. 161 9 ） μg · g- 1 respectively ; the expression of FGFR1 mRNA in heart, muscle, kidney, fat, blood vessel and liver tissue was decreased in turn;the expression of FGFR1 protein in fat, blood vessel, muscle, heart, liver and kidney tissue was decreased in turn ; there were statistic difference of the expression of FGFR1 mRNA and protein in above each tissue of rats （ P 〈 0.05, P 〈 0. 01 ）. Conclusion FGFR1 mRNA and protein expresses in the major tissues of rats, and mainly distribute in the cell membrane and the expression in each tissue has significant difference.