降糖消渴颗粒对糖尿病大鼠脂质代谢及肝脏AMPK信号通路的影响

Effects of Jiangtang Xiaoke Granule on lipid metabolism and AMPK signaling pathway in diabetic rats

目的:观察肝脾肾同调的组方降糖消渴颗粒对糖尿病(DM)大鼠脂代谢的作用,并通过其对腺苷酸活化蛋白激酶(AMPK)信号通路的影响探讨可能的作用机制。方法:SD大鼠高脂饲料喂养4周后结合腹腔注射链脲佐菌素(STZ)30mg/kg,建立2型DM大鼠模型,将成模的大鼠按血糖随机分为模型组、阳性对照组(吡格列酮片1.5mg/kg体质量)、降糖消渴颗粒组(9.0g生药/kg体质量),并设正常对照组。干预4周后,称量大鼠肝重,检测大鼠血清血脂4项总胆固醇(TC),甘油三酯(TG),低密度脂蛋白胆固醇(LDL-C),高密度脂蛋白胆固醇(HDL-C)水平及肝功能相关指标,实时荧光定量PCR法检测大鼠肝脏组织AMPKαm RNA表达,以及AMPKα、p-AMPKα、乙酰辅酶A羧化酶(p-ACC)蛋白表达,观察降糖消渴颗粒对上述指标的影响。结果:降糖消渴颗粒能够使DM大鼠血清TC、TG、LDL-C水平显著下降33%、57%、44%,同时使HDL-C水平显著提高,ALT水平显著降低,与模型组比较,差异具有统计学意义(P<0.05,P<0.01)。与模型组相比,降糖消渴颗粒能显著上调DM大鼠肝脏AMPKαm RNA及蛋白表达,同时提高DM大鼠骨肝脏AMPKα、p-AMPKα及p-ACC蛋白表达量(P<0.05)。结论:降糖消渴颗粒能改善DM大鼠脂代谢,其作用机制可能与调节肝脏AMPK信号通路相有关。

Objective： To study the effect of Jiangtang Xiaoke（JTXK） Granule, which was constructed under the guidance of treating type 2 diabetes by regulating spleen, liver, and kidney together, on liver lipid metabolism and AMPK signal pathway in diabetic rats. Methods： Type 2 diabetic rat models were induced by high-fat diet and low dose of streptozotocin（30mg/kg） for 4 weeks. Then model rats were randomly divided into model group, positive control group（pioglitazone 5mg/kg, and JTXK granule group（9.0g/kg） with the same amount of normal SD rats as the normal control group. After 4 weeks of intervention, the liver weight of rats was weighed and the serum total cholesterol（TC）, triglyceride（TG）, low density lipoprotein cholesterol（LDL-C）, high density lipoprotein cholesterol（ALT, AST） and liver function（ALT, AST） were tested. The expressions of AMPKα, p-AMPKα and p-ACC were examined by real-time fluorescence quantitative PCR（FQ-PCR）, and the effects of Jiangtang Xiaoke Granule on the above indexes were observed. Results： TG, LDL-C levels of DM rats in JTXK granule group decreased by 33%, 57%, 44%, respectively, the level of HDL-C increased significantly, and ALT level decreased significantly, compared with the model group difference was statistically significant（P〈0.05, P〈0.01）. Compared with the model group, Jiangtang Xiaoke Granule could up-regulate the expression of AMPKα m RNA and protein in the liver of DM rats, and increase the expression of p-AMPKα and p-ACC protein in DM rats（P〈0.05）. Conclusion： Jiangtang Xiaoke Granule can improve the lipid metabolism of DM rats, and its mechanism may be related to the regulation of hepatic AMPK signal pathway.