电针刺激头部运动区通过PI3K/Akt信号通路减轻脑瘫鼠脑损伤的研究

Study on electroacupuncture stimulating the motor area of scalp acupuncture for reducing cerebral injury of cerebral palsy rats by PI3K/Akt signaling pathway

目的:观察电针刺激脑瘫鼠头部运动区是否通过调控磷脂酰肌醇3-激酶/蛋白激酶B/(PI3K/Akt/GSK3β)信号通路来促进神经功能恢复。方法:构建缺血缺氧性脑瘫大鼠模型,设立正常组、模型组、干预1、2、3组;通过Western Blot法检测海马组织p-PDK1、Akt、p-Akt、GSK-3β、p-GSK-3β(ser9)、MCL-1表达、组织病理及神经行为学评估进行综合评价。结果:与模型组比较,干预1组、干预3组的运动功能评分在各时段都较高,海马神经细胞凋亡减少,海马组织p-Akt、p-PDK1、p-GSK-3β(ser9)、MCL-1的表达明显;而干预2组与模型组比较各项实验指标均无显著差异。结论:电针刺激头部运动区通过PI3K/Akt/GSK3β信号通路可有效减轻脑瘫鼠脑损伤,促进神经功能恢复。

Objective： To observe whether electroacupuncture stimulates the motor area of scalp acupuncture of cerebral palsy rats would promote the recovery of neurological function by regulating PI3K/Akt/GSK3β signaling pathway. Methods： Hypoxic ischemia cerebral palsy rat models were established, the rats were divided into control group, model group, treatment 1, 2, 3 group. Expression of p-PDK1, Akt, p-Akt, GSK-3β, p-GSK-3β（ser9） and MCL-1 were determined by Western Blot, and comprehensive evaluation were made based on neurobehavioral. Results： Compared with the model group, the motor functionscore in the treatment 1 group and the treatment 3 group were higher at different time points, the apoptosis of hippocampus neurons were decreased, the expression of p-Akt, p-PDK1, p-3β（ser9） and MCL-1 were increased. There was no significant difference between treatment 2 group and model group. Conclusion： Electroacupuncture stimulates the motor area of scalp acupuncture through the PI3K/Akt/GSK3β signaling pathway can reduce brain damage and promote recovery of neurological function in cerebral palsy rats.