基于HSGC-MS技术分析苍耳草与苍耳子挥发性成分差异

Analysis on the difference of volatile components in Herba Xanthii and Fructus Xanthii with HSGC-MS

目的:基于顶空气相色谱-质谱(HSGC-MS)技术,建立了分析不同产地来源的苍耳草和苍耳子中挥发性成分的方法。方法:采用HSGC-MS技术对苍耳草和苍耳子20批样品进行测定,通过NIST05标准质谱检索库对主要化合物进行鉴定,数据处理用SIMCA-P 11.5软件进行主成分分析。结果:主成分得分图显示,苍耳草及苍耳子样品间的差异得到明显区分,通过载荷图和T检验筛选出差异显著的11种标志物丙醛(Propanal,t R/min1.64)、二甲基硫醚(Dimethyl sulfide,t R/min 1.73)、2-甲基丙醛(Propanal,2-methyl-,t R/min 1.83)、正丁醛(Butanal,t R/min 1.95)、2,3-丁二酮(2,3-Butanedione,t R/min 2.07)、2-甲基丁醛(Butanal,2-methyl-,t R/min 2.48)、丙二酸(Propanedioic acid,t R/min 2.74)、1-氯戊烷(Pentane,1-chloro-,t R/min 3.62)、己醛(Hexanal,t R/min 4.62)、庚醛(Heptanal,t R/min 7.97)、a-蒎烯(1R.alpha-pinene,t R/min 9.53)。结论:从苍耳草与苍耳子挥发性成分差异的角度,可为揭示二者药性、药效差异的物质基础提供依据。

Objective： To establish a method to analyze the volatile components in Herba Xanthii and Fructus Xanthii from different sources with HSGC-MS. Methods： Twenty batches of Herba Xanthii and Fructus Xanthii samples were analyzed with HSGC-MS and the NIST05 standard mass spectrum base was used to identify the main compounds. Data were processed with SIMCA-P 11.5 software for the principal component analysis. Results： Principal component score chart showed that the samples of Herba Xanthii and Fructus Xanthii were clearly distinguished and 11 markers with significant differences were obtained with the loading diagram and T test. Conclusion： The difference of the volatile components in Herba Xanthii and Fructus Xanthii can provide some evidence for studying the material basis of the different properties and efficacies of the two herbs.