心血管中枢与心血管功能的同步记录技术

Establishment of simultaneous recording technique for observing cardiovascular center and cardiovascular activities

目的:建立心血管中枢与心血管功能的同步记录技术,用于观察心血管中枢活动和外周自主神经功能活动,研究心血管中枢对心血管活动的调节。方法:取成年SD大鼠,用混合麻药腹腔麻醉后,同步记录延髓头端腹外侧区(rostral ventrolateral medulla,RVLM)神经元单位放电活动、心电图、血压、呼吸肌肌电、体温,并进行相关参数的分析。结果:可记录到明确稳定的神经元单位放电活动等各项指标,在12只大鼠的RVLM中一共记录了18个自发单位放电,其中连续放电有12个,周期性簇状放电有6个。另外,两种类型放电分别对应的同步记录指标进行组间比较,仅呼吸肌肌电幅值差异有统计学意义(P<0.05)。在记录各项指标时观察到RVLM神经元自发放电与血压之间有相互影响。结论:同步记录心血管中枢与心血管活动是一种实时可靠的在体动物实验技术,为研究心血管各级中枢之间的相互作用提供了一种有效的方法。

Objective： To establish a simultaneous recording technique for observing the activities of the cardiovascular center and peripheral autonomic nervous system in studying the regulation of the center on cardiovascular activities.Methods： Adult Sprague-Dawley rats were anesthetized with mixed anesthetic. The single-unit discharge of rostral ventrolateral medulla（ RVLM） neurons,electrocardiogram（ ECG）,blood pressure（ BP）,respiratory muscle electromyogram and body temperature were simultaneously recorded and related parameters were analyzed. Results： Stable and clear single-unit discharge signal of neurons and other signals were well recorded. Eighteen spontaneous unit discharges were recorded in 12 rats,containing 12 continuous discharges and 6 periodical burst discharges. In addition,only the respiratory muscle electromyogram amplitude was significantly different（ P 〈0. 05） between two types of discharge groups. The interaction between the RVLM spontaneous neural discharge and blood pressure was observed when recording the indicators.Conclusion： The results indicate that the simultaneous recording technique is a real-time and reliable method for animal experiment in vivo,which may provide an effective technique for studying the interaction between cardiovascular and all levels of the central nervous system.