多酶定向共固定化的研究进展

Research Progress of Oriented Co-immobilization of Multi-enzyme Reaction Systems

多酶体系的定向共固定可以充分地结合不同种类酶的催化特性,既扩展了酶催化的应用范围,又提高了酶体系的整体反应效率,在很大程度上克服了单酶反应体系单一催化的局限性。文章对单酶应用领域的定向固定化方法作了概述,并讨论了多酶反应体系定向共固定化技术的发展现状。多酶体系定向固定化目前主要依赖于非共价定向化的方法,包括生物亲和作用以及DNA技术。

Oriented co-immobilization of multi-enzyme system can fully combine the catalytic properties of different enzymes, which expands the application range of enzyme catalysis and improves overall reaction efficiency of enzyme system.Thus,it overcomes the limit that single enzyme reaction system can only catalyze one kind of reaction.This paper summarizes the oriented-immobilization methods of single-enzyme reaction and discusses development status of oriented co-immobilization technology of multi-enzyme system.Oriented co-immobilization of multi-enzyme system mainly depends on non-covalent orientation methods,including biological affinity interaction and DNA technology.