桦褐孔菌子实体多酚的分离及抗氧化性的研究

Study on Separation of Polyphenol from Fruit Body of Inonotus obliquus and Its Antioxidant Activity

桦褐孔菌子实体中含有大量的活性多酚,为了对多酚进行有效分离,采用甲醇、乙醇和水作为提取溶剂,通过测定多酚含量和DPPH自由基清除率来比较不同溶剂的提取效率,并运用Sephadex LH-20柱色谱对多酚提取液进行分离。结果表明:在60℃条件下,以甲醇为溶剂并结合超声破碎得到的提取液中多酚含量和DPPH自由基清除率最高,同时Sephadex LH-20柱色谱对乙酸乙酯层和正丁醇层多酚实现了有效初步分离,并得出多酚的组成与抗氧化活性紧密关联。多酚的初步分离为进一步深入研究多酚组分提供了基础。

The fruit body of Inonotus obliquus contains abundant active polyphenols.In order to separate the polyphenols,methanol,ethanol and water were used as the extraction solvents,and the extraction efficiency of different solvents was compared through determining content and DPPH radical scavenging activity of polyphenol.Meanwhile,Sephadex LH-20 column chromatography was applied to separate the polyphenol extract.The study results show that under the condition of 60 ℃ ,the content and DPPH radical scavenging activity of polyphenol in extract reach the maximum when methanol as the solvent is combined with ultrasonication.Besides,good preliminary separation of polyphenol at ethyl acetate layer and n-butyl alcohol layer is achieved by using Sephadex LH-20 column chromatography.The result indicates that polyphenol composition is closely related to antioxidant activity. Preliminary separation of polyphenol lays a foudnaiton for further study of polyphenol components.