大豆5个新发现ERF基因的生物信息学及表达分析

Bioinformatics and expression analysis of 5 newfound ERF genes in soybean

ERF转录因子广泛存在于植物中并且参与植物对生物及非生物胁迫的响应。从NCBI数据库中获得5个新的ERF基因,Gm ERFa/b/c/d/e。蛋白序列分析显示,5个ERF基因均含有一个保守的AP2/ERF结合域。进化分析表明,Gm ERFe与Gm ERF3和Gm ERF7同源性最高,Gm ERFb、Gm ERFc与Gm ERF5的同源性最高,Gm ERFd与Gm EREBP1的同源性较高,而Gm ERFa与其他ERF蛋白的同源性均较低。实时荧光定量PCR结果显示,5个基因都主要在大豆的根和叶中表达。逆境处理后的实时荧光定量PCR结果显示,Gm ERFd/e主要对乙烯信号和低温产生响应,Gm ERFb主要对干旱产生响应,而Gm ERFa主要对低温产生响应。

ERF transcription factors are widespread in plants,which are widely involved in plant response to biotic and abiotic stress. In this study,five novel ERF gene sequences,ERFa/b/c/d/e,were obtained by blast from NCBI data base. Protein sequences analysis showed that the five ERF proteins all contained a typical AP2/ERF binding domain. Phylogenetic analysis indicated that Gm ERFe was highly homologous to Gm ERF3 and Gm ERF7; Gm ERFb and Gm ERFc were highly homologous to Gm ERF5; Gm ERFd was homologous to Gm EREBP1; while Gm ERFa had lower homology with other ERF proteins. The expression patterns of five ERF genes in soybean tissues and under stress treatments were analyzed by real-time quantitative PCR. The results showed that the five ERF genes expressed highly in root and leaf. Gm ERFd/e responded mainly to ethylene signal and low temperature,Gm ERFb responded mainly to drought,and Gm ERFa responded mainly to low temperature.