诃子及其炮制品对小鼠小肠5-羟色胺分泌的影响

Effects of different processed products of Terminalia chebula Retz. on 5-HT of small intestine in mice

为探究诃子的2种藏医特殊炮制品(茜草制诃子和白狼毒制诃子)对小鼠小肠5-羟色胺(5-hydroxytryptamine,5-HT)免疫反应(immunoreactive,IR)细胞分泌活动的影响,采用免疫组织化学方法对小肠内5-HT的细胞定位及蛋白表达进行研究。结果表明,5-HT在小肠各段均有分布,其蛋白表达量从十二指肠到回肠逐渐降低。给药28 d后,茜草制诃子(1.0、4.0 g·kg^(-1))、白狼毒制诃子(1.0、4.0 g·kg^(-1))和原药诃子(4.0 g·kg^(-1))处理组小鼠小肠各段5-HT蛋白表达量均显著减少。药效表现为茜草制诃子>白狼毒制诃子>原药诃子,且4.0 g·kg^(-1)剂量的效果优于1.0 g·kg^(-1)。5-HT-IR细胞形态多样,主要呈锥体形、椭圆形、梭形等。对照组5-HT-IR细胞主要分布在肠腺上皮和肠固有膜,而试验组在肠黏膜上皮细胞之间和肠腺上皮分布多一些。由此可知,诃子及其白狼毒制、茜草制炮制品能不同程度地抑制小肠5-HT的分泌,其中以4.0 g·kg^(-1)的茜草制诃子效果最好。

This study investigated the effects of Terminalia chebula Retz. and its 2 processed products of Tibetan medicine,Rubia cordifolia L. per Terminalia chebula and Euphorbia fischeriana Steud per Terminalia chebula on the secretory activity of 5-hydroxytryptamine immunoreactive（ 5-HT-IR） cells in small intestine of mice. Cellular localization and protein expression of 5-HT-IR cells in small intestine were revealed by immunohistochemistry. It was shown that 5-HT cells were widely distributed in small intestine,and the protein expression gradually reduced from duodenum to ileum. After drug delivery for 28 d,the protein expression of 5-HT of small intestine in mice was significantly lower in Rubia cordifolia L. per Terminalia chebula groups（ in the dosage of 1. 0 and 4. 0 g·kg^-1）,Euphorbia fischeriana Steud per Terminalia chebula groups（ in the dosage of 1. 0 and 4. 0 g·kg^-1） and Terminalia chebula group（ in the dosage of 4. 0 g·kg^-1）. The efficacy decreased as Rubia cordifolia L. per Terminalia chebula〉 Euphorbia fischeriana Steud per Terminalia chebula〉 Terminalia chebula. The efficacies of Terminalia chebula and itsprocessed products in dosage of 4. 0 g·kg^-1were better than those in dosage of 1. 0 g·kg^-1. The shape of 5-HT cells varied as cone,ellipse,and spindle. A large number of 5-HT-IR cells were found in the epithelial of intestinal gland and intestinal lamina propria in control group,while these cells were widely distributed in the epithelial cells of intestinal mucosal and epithelial of intestinal gland in experimental groups. This study indicated that Terminalia chebula and its processed products played an important role in adjusting physiological function of small intestine by inhibiting the secretion of 5-HT,and the effect of Rubia cordifolia L. per Terminalia chebula in the dosage of 4. 0 g·kg^-1was the best.